Universität zu Köln

Liessem, Sander ORCID: 0000-0002-7073-2659, Ragionieri, Lapo, Neupert, Susanne, Bueschges, Ansgar and Predel, Reinhard (2018). Transcriptomic and Neuropeptidomic Analysis of the Stick Insect, Carausius morosus. J. Proteome Res., 17 (6). S. 2192 - 2205. WASHINGTON: AMER CHEMICAL SOC. ISSN 1535-3907

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Abstract

One of the most thoroughly studied insect species, with respect to locomotion behavior, is the stick insect Carausius morosus. Although detailed information exists on premotor networks controlling walking, surprisingly little is known about neuropeptides, which are certainly involved in motor activity generation and modulation. So far, only few neuropeptides were identified from C. morosus or related stick insects. We performed a transcriptome analysis of the central nervous system to assemble and identify 65 neuropeptide and protein hormone precursors of C. morosus, including five novel putative neuropeptide precursors without clear homology to known neuropeptide precursors of other insects (Carausius neuropeptide-like precursor 1, HanSolin, PK-likel, PK-like2, RFLamide). Using Q Exactive Orbitrap and MALDI-TOF mass spectrometry, 277 peptides including 153 likely bioactive mature neuropeptides were confirmed. Peptidomics yielded a complete coverage for many of the neuropeptide propeptides and confirmed a surprisingly high number of heterozygous sequences. Few neuropeptide precursors commonly occurring in insects, including those of insect kinins and sulfakinins, could neither be found in the transcriptome data nor did peptidomics support their presence. The results of our study represent one of the most comprehensive peptidomic analyses on insects and provide the necessary input for subsequent experiments revealing neuropeptide function in greater detail.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Liessem, Sander UNSPECIFIED orcid.org/0000-0002-7073-2659 UNSPECIFIED Ragionieri, Lapo UNSPECIFIED UNSPECIFIED UNSPECIFIED Neupert, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Bueschges, Ansgar UNSPECIFIED UNSPECIFIED UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-183544
DOI:	10.1021/acs.jproteome.8b00155
Journal or Publication Title:	J. Proteome Res.
Volume:	17
Number:	6
Page Range:	S. 2192 - 2205
Date:	2018
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1535-3907
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RNA-SEQ DATA; PROTEIN HORMONES; NONSPIKING INTERNEURONS; LOCUSTA-MIGRATORIA; CORPUS CARDIACUM; AKH/RPCH FAMILY; NERVOUS-SYSTEM; MOTOR-ACTIVITY; JOINT-CONTROL; IDENTIFICATION Multiple languages Biochemical Research Methods Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18354