Universität zu Köln

Goebel, Ulrike, Arce, Agustin L., He, Fei, Rico, Alain, Schmitz, Gregor and de Meaux, Juliette ORCID: 0000-0002-2942-4750 (2018). Robustness of Transposable Element Regulation but No Genomic Shock Observed in Interspecific Arabidopsis Hybrids. Genome Biol. Evol., 10 (6). S. 1403 - 1416. OXFORD: OXFORD UNIV PRESS. ISSN 1759-6653

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Abstract

The merging of two divergent genomes in a hybrid is believed to trigger a genomic shock, disrupting gene regulation and transposable element (TE) silencing. Here, we tested this expectation by comparing the pattern of expression of transposable elements in their native and hybrid genomic context. For this, we sequenced the transcriptome of the Arabidopsis thaliana genotype Col-0, the A. lyrata genotype MN47 and their Fl hybrid. Contrary to expectations, we observe that the level of TE expression in the hybrid is strongly correlated to levels in the parental species. We detect that at most 1.1% of expressed transposable elements belonging to two specific subfamilies change their expression level upon hybridization. Most of these changes, however, are of small magnitude. We observe that the few hybrid-specific modifications in TE expression are more likely to occur when TE insertions are close to genes. In addition, changes in epigenetic histone marks H3K9me2 and H3K27me3 following hybridization do not coincide with TEs with changed expression. Finally, we further examined TE expression in parents and hybrids exposed to severe dehydration stress. Despite the major reorganization of gene and TE expression by stress, we observe that hybridization does not lead to increased disorganization of TE expression in the hybrid. Although our study did not examine TE transposition activity in hybrids, the examination of the transcriptome shows that TE expression is globally robust to hybridization. The term genomic shock is perhaps not appropriate to describe transcriptional modification in a viable hybrid merging divergent genomes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Goebel, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED Arce, Agustin L. UNSPECIFIED UNSPECIFIED UNSPECIFIED He, Fei UNSPECIFIED UNSPECIFIED UNSPECIFIED Rico, Alain UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmitz, Gregor UNSPECIFIED UNSPECIFIED UNSPECIFIED de Meaux, Juliette UNSPECIFIED orcid.org/0000-0002-2942-4750 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-183993
DOI:	10.1093/gbe/evy095
Journal or Publication Title:	Genome Biol. Evol.
Volume:	10
Number:	6
Page Range:	S. 1403 - 1416
Date:	2018
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1759-6653
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DIFFERENTIAL EXPRESSION ANALYSIS; TRAGOPOGON-MISCELLUS ASTERACEAE; GENE-EXPRESSION; SMALL RNAS; CHIP-SEQ; TRANSCRIPTOME SHOCK; BRASSICA-RAPA; HIGH-SALINITY; HYBRIDIZATION; EVOLUTION Multiple languages Evolutionary Biology; Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18399