Universität zu Köln

Solbach, Philipp ORCID: 0000-0003-2708-3611, Chhatwal, Patrick, Woltemate, Sabrina, Tacconelli, Evelina, Buhl, Michael, Gerhard, Markus ORCID: 0000-0001-9110-3950, Thoeringer, Christoph K., Vehreschild, Maria J. G. T., Jazmati, Nathalie, Rupp, Jan, Manns, Michael P., Bachmann, Oliver ORCID: 0000-0002-6830-2975 and Suerbaum, Sebastian (2018). BaiCD gene cluster abundance is negatively correlated with Clostridium difficile infection. PLoS One, 13 (5). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Background Clostridium difficile infection (CDI) is a major cause of hospital-acquired diarrhea. Secondary bile acids were shown to confer resistance to colonization by C. difficile. 7 alpha-dehydroxylation is a key step in transformation of primary to secondary bile acids and required genes have been located in a single bile acid-inducible (bai) operon in C. scindens as well as in C. hiranonis, two Clostridium sp. recently reported to protect against C. difficile colonization. Aim To analyze baiCD gene abundance in C. difficile positive and negative fecal samples. Material & methods A species-specific qPCR for detecting baiCD genes was established. Fecal samples of patients with CDI, asymptomatic toxigenic C. difficile colonization (TCD), non-toxigenic C. difficile colonization (NTCD), of C. difficile negative (NC) patients, and of two patients before and after fecal microbiota transplantation (FMT) for recurrent CDI (rCDI) were tested for the presence of the baiCD genes. Results The prevalence of the baiCD gene cluster was significantly higher in C. difficile negative fecal samples than in samples of patients diagnosed with CDI (72.5% (100/138) vs. 35.9% (23/64; p<0.0001). No differences in baiCD gene cluster prevalence were seen between NC and NTCD or NC and TCD samples. Both rCDI patients were baiCD-negative at baseline, but one of the two patients turned positive after successful FMT from a baiCD-positive donor. Conclusion Fecal samples of CDI patients are less frequently baiCD-positive than samples from asymptomatic carriers or C. difficile-negative individuals. Furthermore, we present a case of baiCD positivity observed after successful FMT for rCDI.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Solbach, Philipp UNSPECIFIED orcid.org/0000-0003-2708-3611 UNSPECIFIED Chhatwal, Patrick UNSPECIFIED UNSPECIFIED UNSPECIFIED Woltemate, Sabrina UNSPECIFIED UNSPECIFIED UNSPECIFIED Tacconelli, Evelina UNSPECIFIED UNSPECIFIED UNSPECIFIED Buhl, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerhard, Markus UNSPECIFIED orcid.org/0000-0001-9110-3950 UNSPECIFIED Thoeringer, Christoph K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vehreschild, Maria J. G. T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jazmati, Nathalie UNSPECIFIED UNSPECIFIED UNSPECIFIED Rupp, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Manns, Michael P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bachmann, Oliver UNSPECIFIED orcid.org/0000-0002-6830-2975 UNSPECIFIED Suerbaum, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-186596
DOI:	10.1371/journal.pone.0196977
Journal or Publication Title:	PLoS One
Volume:	13
Number:	5
Date:	2018
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REAL-TIME PCR; FECAL MICROBIOTA TRANSPLANTATION; ACID 7-ALPHA-DEHYDROXYLATING ACTIVITY; BILE-SALT BIOTRANSFORMATIONS; GUT MICROBIOTA; INTESTINAL MICROBIOTA; COLONIZATION RESISTANCE; MULTICENTER EVALUATION; ASYMPTOMATIC CARRIAGE; HOSPITALIZED-PATIENTS Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18659