Universität zu Köln

Kalbe, Benjamin, Osterloh, Markus, Schulz, Viola M., Altmueller, Janine, Becker, Christian, Osterloh, Sabrina and Hatt, Hanns (2018). OR2H2 regulates the differentiation of human myoblast cells by its ligand aldehyde 13-13. Arch. Biochem. Biophys., 645. S. 72 - 81. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1096-0384

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Abstract

Olfactory receptors (ORs) regulate various cellular processes in the human body. The receptors' participation in physiological and pathophysiological processes could be demonstrated in several studies. In addition to the regulation of sperm motility, respiratory physiology, and heart contraction, ORs play a crucial role in cancer cells. In murine myoblasts, mOR23 regulates the myogenesis and branching of skeletal muscle cells. To date, the expression and physiological role of ORs in human skeletal muscle cells have not been thoroughly elucidated. We demonstrate that four different ORs are expressed at the transcript level in differentiated myoblasts, and one other OR is expressed in undifferentiated myoblasts. Moreover, we characterized the expression of OR2H2 in differentiated human myoblasts and identified a specific ligand, aldehyde 13-13. We could observe a concentration-dependent Ca2+ increase in differentiated human myoblasts upon aldehyde 13-13 stimulation, which is mediated by PI3K signaling. Aldehyde 13-13 has a reducing effect on myoblast fusion. We conclude that OR2H2 could have a regulatory role in myoblast differentiation. To the best of our knowledge, this report presents the first verification of the expression of ORs in human myoblasts. OR2H2 might be an interesting candidate for playing a role in the complex mechanism of myogenesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kalbe, Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Osterloh, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulz, Viola M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Osterloh, Sabrina UNSPECIFIED UNSPECIFIED UNSPECIFIED Hatt, Hanns UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-188394
DOI:	10.1016/j.abb.2018.03.017
Journal or Publication Title:	Arch. Biochem. Biophys.
Volume:	645
Page Range:	S. 72 - 81
Date:	2018
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1096-0384
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INHIBITS PROLIFERATION; MUSCLE REGENERATION; ODORANT RECEPTORS; CALCIUM INFLUX; GROWTH-FACTOR; ACTIVATION; MIGRATION; CHANNELS; QUANTIFICATION; IDENTIFICATION Multiple languages Biochemistry & Molecular Biology; Biophysics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18839