Universität zu Köln

Hipolito, Jolly, Peretz-Soroka, Hagit, Torres, Aniel Moya, Booy, Evan, Yang, Ke, Gupta, Monika, Meier, Markus ORCID: 0000-0003-1068-746X, McKenna, Sean, Koch, Manuel, Santos, Susy, Stetefeld, Jorg and Lin, Francis (2018). Microfluidic Devices for Studying the Effect of Netrinon-1 Neutrophil and Breast Cancer Cell Migration. Adv. Biosyst., 2 (4). WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 2366-7478

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Abstract

Netrin-1 is well-known for its chemoattractive and chemorepulsive properties for axon guidance. Early studies report that netrin-1 inhibits granulocyte migration. On the other hand, netrin-1 can promote cancer cell migration and invasion. The underlying mechanisms are not well understood, which requires more in-depth characterizations of netrin-1 mediated immune and cancer cell migration. The present study, for the first time, employs microfluidic devices that are recently developed to quantitatively investigate the effects of netrin-1 on the motility and chemotaxis of human blood neutrophils and human breast cancer cells under well-controlled gradient conditions. The results show that netrin-1 reduces chemokinetic motility of human neutrophils, which is accompanied with reduced cell polarization and spreading. In addition, netrin-1 reduces neutrophil chemotaxis to N-formyl-Met-Leu-Phe on fibronectin substrate but interestingly not on collagen substrate. By contrast, netrin-1 promotes the migration of human breast cancer cells. Furthermore, it is found that netrin-1 reduces neutrophil chemotaxis to the supernatant of human breast cancer cell culture. Collectively, this microfluidic cell migration study provides quantitative characterizations of the effects of netrin-1 on the motility and chemotaxis of neutrophils and breast cancer cells, and further suggests the potential role of netrin-1 in regulating neutrophil recruitment to breast cancer microenvironments.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hipolito, Jolly UNSPECIFIED UNSPECIFIED UNSPECIFIED Peretz-Soroka, Hagit UNSPECIFIED UNSPECIFIED UNSPECIFIED Torres, Aniel Moya UNSPECIFIED UNSPECIFIED UNSPECIFIED Booy, Evan UNSPECIFIED UNSPECIFIED UNSPECIFIED Yang, Ke UNSPECIFIED UNSPECIFIED UNSPECIFIED Gupta, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Meier, Markus UNSPECIFIED orcid.org/0000-0003-1068-746X UNSPECIFIED McKenna, Sean UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Santos, Susy UNSPECIFIED UNSPECIFIED UNSPECIFIED Stetefeld, Jorg UNSPECIFIED UNSPECIFIED UNSPECIFIED Lin, Francis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-189792
DOI:	10.1002/adbi.201700178
Journal or Publication Title:	Adv. Biosyst.
Volume:	2
Number:	4
Date:	2018
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	2366-7478
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VITRO; CHEMOTAXIS; METASTASIS; FMLP; CCR7 Multiple languages Materials Science, Biomaterials Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18979