Universität zu Köln

Diesner, Max, Gallot, Aurore, Binz, Hellena, Gaertner, Cyril, Vitecek, Simon ORCID: 0000-0002-7637-563X, Kahnt, Joerg, Schachtner, Joachim ORCID: 0000-0001-7268-0699, Jacquin-Joly, Emmanuelle ORCID: 0000-0002-6904-2036 and Gadenne, Christophe (2018). Mating-Induced Differential Peptidomics of Neuropeptides and Protein Hormones in Agrotis ipsilon Moths. J. Proteome Res., 17 (4). S. 1397 - 1415. WASHINGTON: AMER CHEMICAL SOC. ISSN 1535-3907

Full text not available from this repository.

Abstract

In many insects, mating induces drastic changes in male and female responses to sex pheromones or host-plant odors. In the male moth Agrotis ipsilon, mating induces a transient inhibition of behavioral and neuronal responses to the female sex pheromone. As neuropeptides and peptide hormones regulate most behavioral processes, we hypothesize that they could be involved in this mating-dependent olfactory plasticity. Here we used next-generation RNA sequencing and a combination of liquid chromatography, matrix assisted laser CC desorption ionization time-of-flight (MALDI-TOF) mass and direct tissue profiling to analyze the transcriptome and peptidome of different brain compartments in virgin and mated males and females of A. ipsilon. We identified 37 transcripts encoding putative neuropeptide precursors and 54 putative bioactive neuropeptides from 23 neuropeptide precursors (70 sequences in total, 25 neuropeptide precursors) in different areas of the central nervous system including the antennal lobes, the gnathal ganglion, and the corpora cardiaca-corpora allata complex. Comparisons between virgin and mated males and females revealed tissue-specific differences in peptide composition between sexes and according to physiological state. Mated males showed postmating differences in neuropeptide occurrence, which could participate in the mating-induced olfactory plasticity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Diesner, Max UNSPECIFIED UNSPECIFIED UNSPECIFIED Gallot, Aurore UNSPECIFIED UNSPECIFIED UNSPECIFIED Binz, Hellena UNSPECIFIED UNSPECIFIED UNSPECIFIED Gaertner, Cyril UNSPECIFIED UNSPECIFIED UNSPECIFIED Vitecek, Simon UNSPECIFIED orcid.org/0000-0002-7637-563X UNSPECIFIED Kahnt, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Schachtner, Joachim UNSPECIFIED orcid.org/0000-0001-7268-0699 UNSPECIFIED Jacquin-Joly, Emmanuelle UNSPECIFIED orcid.org/0000-0002-6904-2036 UNSPECIFIED Gadenne, Christophe UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-190098
DOI:	10.1021/acs.jproteome.7b00779
Journal or Publication Title:	J. Proteome Res.
Volume:	17
Number:	4
Page Range:	S. 1397 - 1415
Date:	2018
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1535-3907
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADULT DROSOPHILA-MELANOGASTER; BEETLE TRIBOLIUM-CASTANEUM; SEX-PHEROMONE PRODUCTION; JUVENILE-HORMONE; ANTENNAL LOBES; MANDUCA-SEXTA; SPODOPTERA-LITTORALIS; HELIOTHIS-VIRESCENS; MASS-SPECTROMETRY; NERVOUS-SYSTEM Multiple languages Biochemical Research Methods Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19009