Universität zu Köln

Bukovska, Petra, Bonkowski, Michael, Konvalinkova, Tereza ORCID: 0000-0003-2271-6408, Beskid, Olena, Hujslova, Martina, Puschel, David ORCID: 0000-0002-1949-3242, Rezacova, Veronika ORCID: 0000-0002-1749-0355, Semiramis Gutierrez-Nunez, Maria, Gryndler, Milan and Jansa, Jan ORCID: 0000-0002-0331-1774 (2018). Utilization of organic nitrogen by arbuscular mycorrhizal fungi-is there a specific role for protists and ammonia oxidizers? Mycorrhiza, 28 (3). S. 269 - 284. NEW YORK: SPRINGER. ISSN 1432-1890

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Abstract

Arbuscular mycorrhizal (AM) fungi can significantly contribute to plant nitrogen (N) uptake from complex organic sources, most likely in concert with activity of soil saprotrophs and other microbes releasing and transforming the N bound in organic forms. Here, we tested whether AM fungus (Rhizophagus irregularis) extraradical hyphal networks showed any preferences towards certain forms of organic N (chitin of fungal or crustacean origin, DNA, clover biomass, or albumin) administered in spatially discrete patches, and how the presence of AM fungal hyphae affected other microbes. By direct N-15 labeling, we also quantified the flux of N to the plants (Andropogon gerardii) through the AM fungal hyphae from fungal chitin and from clover biomass. The AM fungal hyphae colonized patches supplemented with organic N sources significantly more than those receiving only mineral nutrients, organic carbon in form of cellulose, or nothing. Mycorrhizal plants grew 6.4-fold larger and accumulated, on average, 20.3-fold more N-15 originating from the labeled organic sources than their nonmycorrhizal counterparts. Whereas the abundance of microbes (bacteria, fungi, or Acanthamoeba sp.) in the different patches was primarily driven by patch quality, we noted a consistent suppression of the microbial abundances by the presence of AM fungal hyphae. This suppression was particularly strong for ammonia oxidizing bacteria. Our results indicate that AM fungi successfully competed with the other microbes for free ammonium ions and suggest an important role for the notoriously understudied soil protists to play in recycling organic N from soil to plants via AM fungal hyphae.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bukovska, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonkowski, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Konvalinkova, Tereza UNSPECIFIED orcid.org/0000-0003-2271-6408 UNSPECIFIED Beskid, Olena UNSPECIFIED UNSPECIFIED UNSPECIFIED Hujslova, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Puschel, David UNSPECIFIED orcid.org/0000-0002-1949-3242 UNSPECIFIED Rezacova, Veronika UNSPECIFIED orcid.org/0000-0002-1749-0355 UNSPECIFIED Semiramis Gutierrez-Nunez, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Gryndler, Milan UNSPECIFIED UNSPECIFIED UNSPECIFIED Jansa, Jan UNSPECIFIED orcid.org/0000-0002-0331-1774 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-190145
DOI:	10.1007/s00572-018-0825-0
Journal or Publication Title:	Mycorrhiza
Volume:	28
Number:	3
Page Range:	S. 269 - 284
Date:	2018
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-1890
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SOIL MICROBIAL COMMUNITY; REAL-TIME PCR; LITTER DECOMPOSITION; BACTERIAL COMMUNITY; PLANT-GROWTH; NITRIFICATION RATES; ANDROPOGON-GERARDII; GLOMUS-INTRARADICES; PROTOZOA; CARBON Multiple languages Mycology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19014