Universität zu Köln

Monne, Magnus ORCID: 0000-0003-2344-3878, Daddabbo, Lucia, Gagneul, David, Obata, Toshihiro ORCID: 0000-0001-8931-7722, Hielscher, Bjoern, Palmieri, Luigi, Miniero, Daniela Valeria, Fernie, Alisdair R., Weber, Andreas P. M. and Palmieri, Ferdinando (2018). Uncoupling proteins 1 and 2 (UCP1 and UCP2) from Arabidopsis thaliana are mitochondrial transporters of aspartate, glutamate, and dicarboxylates. J. Biol. Chem., 293 (11). S. 4213 - 4228. BETHESDA: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC. ISSN 1083-351X

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Abstract

The Arabidopsis thaliana genome contains 58 members of the solute carrier family SLC25, also called the mitochondrial carrier family, many of which have been shown to transport specific metabolites, nucleotides, and cofactors across the mitochondrial membrane. Here, two Arabidopsis members of this family, AtUCP1 and AtUCP2, which were previously thought to be uncoupling proteins and hence named UCP1/PUMP1 and UCP2/PUMP2, respectively, are assigned with a novel function. They were expressed in bacteria, purified, and reconstituted in phospholipid vesicles. Their transport properties demonstrate that they transport amino acids (aspartate, glutamate, cysteine sulfinate, and cysteate), dicarboxylates (malate, oxaloacetate, and 2-oxoglutarate), phosphate, sulfate, and thiosulfate. Transport was saturable and inhibited by mercurials and other mitochondrial carrier inhibitors to various degrees. AtUCP1 and AtUCP2 catalyzed a fast counterexchange transport as well as a low uniport of substrates, with transport rates of AtUCP1 being much higher than those of AtUCP2 in both cases. The aspartate/glutamate heteroexchange mediated by AtUCP1 and AtUCP2 is electroneutral, in contrast to that mediated by the mammalian mitochondrial aspartate glutamate carrier. Furthermore, both carriers were found to be targeted to mitochondria. Metabolite profiling of single and double knockouts shows changes in organic acid and amino acid levels. Notably, AtUCP1 and AtUCP2 are the first reported mitochondrial carriers in Arabidopsis to transport aspartate and glutamate. It is proposed that the primary function of AtUCP1 and AtUCP2 is to catalyze an aspartate(out)/glutamate(in) exchange across the mitochondrial membrane and thereby contribute to the export of reducing equivalents from the mitochondria in photorespiration.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Monne, Magnus UNSPECIFIED orcid.org/0000-0003-2344-3878 UNSPECIFIED Daddabbo, Lucia UNSPECIFIED UNSPECIFIED UNSPECIFIED Gagneul, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Obata, Toshihiro UNSPECIFIED orcid.org/0000-0001-8931-7722 UNSPECIFIED Hielscher, Bjoern UNSPECIFIED UNSPECIFIED UNSPECIFIED Palmieri, Luigi UNSPECIFIED UNSPECIFIED UNSPECIFIED Miniero, Daniela Valeria UNSPECIFIED UNSPECIFIED UNSPECIFIED Fernie, Alisdair R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Palmieri, Ferdinando UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-192649
DOI:	10.1074/jbc.RA117.000771
Journal or Publication Title:	J. Biol. Chem.
Volume:	293
Number:	11
Page Range:	S. 4213 - 4228
Date:	2018
Publisher:	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Place of Publication:	BETHESDA
ISSN:	1083-351X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADENINE-NUCLEOTIDE TRANSPORTER; CARRIER FAMILY 25; BASIC-AMINO-ACIDS; BACTERIAL EXPRESSION; FUNCTIONAL-CHARACTERIZATION; SACCHAROMYCES-CEREVISIAE; MALATE-DEHYDROGENASE; TISSUE DISTRIBUTION; ORGAN DISTRIBUTION; COENZYME-A Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19264