Universität zu Köln

Yang, Peng-Fei, Nie, Xiao-Tong, Zhao, Dong-Dong, Wang, Zhe, Ren, Li, Xu, Hui-Yun, Rittvveger, Joern and Shang, Peng (2018). Deformation regimes of collagen fibrils in cortical bone revealed by in situ morphology and elastic modulus observations under mechanical loading. J. Mech. Behav. Biomed. Mater., 79. S. 115 - 122. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1878-0180

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Abstract

The mechanical properties of the bone play a decisive role in the resistance of the bone to fracture. Clinically, the quantity of the bone in the mineral phase has been considered as the gold-standard indicator for the risk of bone fracture. However, the bone is a complex tissue with a hierarchical-structure consisting of organic matrix, mineral hydroxyapatite, and water. Collagen comprises up to 90% of the organic matrix in the bone, and is vital for its mechanical behavior. To date, the morphological and mechanical responses of collagen fibrils in the bone matrix have been largely overlooked. In the present study, an atomic force microscopy-based imaging and indentation approach is introduced and integrated with a tibia axial loading model. The morphology of mineralized Type I collagen fibrils of the murine cortical tibia is imaged after demineralization, and the in situ elastic modulus of the fibrils is quantified at different loading conditions. Results suggested that the mineralized collagen fibrils are stretched in the early phase of bone deformation, characterized by the elongation of the D periodic spacing. Reorientation of the collagen fibrils is demonstrated in the subsequent phase of bone deformation. The in situ radial elastic modulus of the collagen fibrils remained constant under the tested loading conditions. These experimental findings provide evidence in support of the unique deformation regimes of bone tissue from the perspective of alterations of mineralized collagen fibrils. This study allows the understanding of the unique mechanical behavior of the bone at the nanoscale, and reveals the mechanisms of relevant diseases that impair the mechanical properties of the bone.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Yang, Peng-Fei UNSPECIFIED UNSPECIFIED UNSPECIFIED Nie, Xiao-Tong UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhao, Dong-Dong UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Zhe UNSPECIFIED UNSPECIFIED UNSPECIFIED Ren, Li UNSPECIFIED UNSPECIFIED UNSPECIFIED Xu, Hui-Yun UNSPECIFIED UNSPECIFIED UNSPECIFIED Rittvveger, Joern UNSPECIFIED UNSPECIFIED UNSPECIFIED Shang, Peng UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-195197
DOI:	10.1016/j.jmbbm.2017.12.015
Journal or Publication Title:	J. Mech. Behav. Biomed. Mater.
Volume:	79
Page Range:	S. 115 - 122
Date:	2018
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1878-0180
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language VISCOELASTIC PROPERTIES; NANOSCALE MORPHOLOGY; MINERAL-CONTENT; MOUSE MODEL; ORIENTATION; ORGANIZATION; STRENGTH; TISSUE; INDENTATION; LAMELLAE Multiple languages Engineering, Biomedical; Materials Science, Biomaterials Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19519