Universität zu Köln

Matei, Alexandra, Ernst, Corinna, Guenl, Markus, Thiele, Bjoern, Altmueller, Janine, Walbot, Virginia, Usadel, Bjoern and Doehlemann, Gunther ORCID: 0000-0002-7353-8456 (2018). How to make a tumour: cell type specific dissection of Ustilago maydis-induced tumour development in maize leaves. New Phytol., 217 (4). S. 1681 - 1696. HOBOKEN: WILEY. ISSN 1469-8137

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Abstract

The biotrophic fungus Ustilago maydis causes smut disease on maize (Zea mays), which is characterized by immense plant tumours. To establish disease and reprogram organ primordia to tumours, U. maydis deploys effector proteins in an organ-specific manner. However, the cellular contribution to leaf tumours remains unknown. We investigated leaf tumour formation at the tissue-and cell type-specific levels. Cytology and metabolite analysis were deployed to understand the cellular basis for tumourigenesis. Laser-capture microdissection was performed to gain a cell type-specific transcriptome of U. maydis during tumour formation. In vivo visualization of plant DNA synthesis identified bundle sheath cells as the origin of hyperplasic tumour cells, while mesophyll cells become hypertrophic tumour cells. Cell typespecific transcriptome profiling of U. maydis revealed tailored expression of fungal effector genes. Moreover, U. maydis See1 was identified as the first cell type-specific fungal effector, being required for induction of cell cycle reactivation in bundle sheath cells. Identification of distinct cellular mechanisms in two different leaf cell types and of See1 as an effector for induction of proliferation of bundle sheath cells are major steps in understanding U. maydis-induced tumour formation. Moreover, the cell type-specific U. maydis transcriptome data are a valuable resource to the scientific community.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Matei, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Ernst, Corinna UNSPECIFIED UNSPECIFIED UNSPECIFIED Guenl, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Thiele, Bjoern UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Walbot, Virginia UNSPECIFIED UNSPECIFIED UNSPECIFIED Usadel, Bjoern UNSPECIFIED UNSPECIFIED UNSPECIFIED Doehlemann, Gunther UNSPECIFIED orcid.org/0000-0002-7353-8456 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-195370
DOI:	10.1111/nph.14960
Journal or Publication Title:	New Phytol.
Volume:	217
Number:	4
Page Range:	S. 1681 - 1696
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-8137
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPRESSION ANALYSIS; PLANT DEFENSE; SMUT FUNGUS; INFECTION; GENE; ENDOREDUPLICATION; CHLOROPLASTS; ACTIVATION; VIRULENCE; SEEDLINGS Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19537