Universität zu Köln

Lanver, Daniel, Mueller, Andre N., Happel, Petra, Schweizer, Gabriel ORCID: 0000-0002-8679-4598, Haas, Fabian B., Franitza, Marek, Pellegrin, Clement ORCID: 0000-0002-6591-655X, Reissmann, Stefanie, Altmueller, Janine, Rensing, Stefan A. and Kahmann, Regine (2018). The Biotrophic Development of Ustilago maydis Studied by RNA-Seq Analysis. Plant Cell, 30 (2). S. 300 - 324. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-298X

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Abstract

The maize smut fungus Ustilago maydis is a model organism for elucidating host colonization strategies of biotrophic fungi. Here, we performed an in depth transcriptional profiling of the entire plant-associated development of U. maydis wild-type strains. In our analysis, we focused on fungal metabolism, nutritional strategies, secreted effectors, and regulatory networks. Secreted proteins were enriched in three distinct expression modules corresponding to stages on the plant surface, establishment of biotrophy, and induction of tumors. These modules are likely the key determinants for U. maydis virulence. With respect to nutrient utilization, we observed that expression of several nutrient transporters was tied to these virulence modules rather than being controlled by nutrient availability. We show that oligopeptide transporters likely involved in nitrogen assimilation are important virulence factors. By measuring the intramodular connectivity of transcription factors, we identified the potential drivers for the virulence modules. While known components of the b-mating type cascade emerged as inducers for the plant surface and biotrophy module, we identified a set of yet uncharacterized transcription factors as likely responsible for expression of the tumor module. We demonstrate a crucial role for leaf tumor formation and effector gene expression for one of these transcription factors.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lanver, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Andre N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Happel, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Schweizer, Gabriel UNSPECIFIED orcid.org/0000-0002-8679-4598 UNSPECIFIED Haas, Fabian B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Franitza, Marek UNSPECIFIED UNSPECIFIED UNSPECIFIED Pellegrin, Clement UNSPECIFIED orcid.org/0000-0002-6591-655X UNSPECIFIED Reissmann, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Rensing, Stefan A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kahmann, Regine UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-196679
DOI:	10.1105/tpc.17.00764
Journal or Publication Title:	Plant Cell
Volume:	30
Number:	2
Page Range:	S. 300 - 324
Date:	2018
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-298X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GLOBAL NITROGEN REGULATOR; FUNGAL PLANT-PATHOGENS; GENE-EXPRESSION DATA; SACCHAROMYCES-CEREVISIAE; CANDIDA-ALBICANS; TRANSCRIPTOME ANALYSES; NUTRIENT ACQUISITION; ZYMOSEPTORIA-TRITICI; FILAMENTOUS GROWTH; SECRETED EFFECTOR Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19667