Universität zu Köln

Ordonez-Herrera, Natalia, Trimborn, Laura, Menje, Melanie, Henschel, Monique, Robers, Lennart, Kaufholdt, David ORCID: 0000-0001-7509-2833, Hansch, Robert, Adrian, Jessika, Ponnu, Jathish ORCID: 0000-0002-3276-7068 and Hoecker, Ute ORCID: 0000-0002-5636-9777 (2018). The Transcription Factor COL12 Is a Substrate of the COP1/SPA E3 Ligase and Regulates Flowering Time and Plant Architecture. Plant Physiol., 176 (2). S. 1327 - 1341. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

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Abstract

The ambient light environment controls many aspects of plant development throughout a plant's life cycle. Such complex control is achieved because a key repressor of light signaling, the Arabidopsis (Arabidopsis thaliana) COP1/SPA E3 ubiquitin ligase causes the degradation of multiple regulators of endogenous developmental pathways. This includes the CONSTANS (CO) transcription factor that is responsible for photoperiodic control of flowering time. There are 16 CO-like proteins whose functions are only partly understood. Here, we show that 14 CO-like (COL) proteins bind CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1) and SUPPRESSOR OF PHYTOCHROME A-105 (SPA) 1 in vitro. We subsequently focused on COL12 and show that COL12 binds COP1 and SPA proteins in vivo. The COL12 protein is degraded in darkness in a COP1-dependent fashion, indicating that COL12 is a substrate of the COP1/SPA ubiquitin ligase. Overexpression of COL12 causes late flowering specifically in long day conditions by decreasing the expression of FLOWERING LOCUS T. This phenotype is genetically dependent on CO. Consistent with this finding, COL12 physically interacts with CO in vivo, suggesting that COL12 represses flowering by inhibiting CO protein function. We show that COL12 overexpression did not alter CO protein stability. It is therefore likely that COL12 represses the activity of CO rather than CO levels. Overexpression of COL12 also affects plant architecture by increasing the number of rosette branches and reducing inflorescence height. These phenotypes are CO independent. Hence, we suggest that COL12 affects plant development through CO-dependent and CO-independent mechanisms.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ordonez-Herrera, Natalia UNSPECIFIED UNSPECIFIED UNSPECIFIED Trimborn, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Menje, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Henschel, Monique UNSPECIFIED UNSPECIFIED UNSPECIFIED Robers, Lennart UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaufholdt, David UNSPECIFIED orcid.org/0000-0001-7509-2833 UNSPECIFIED Hansch, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Adrian, Jessika UNSPECIFIED UNSPECIFIED UNSPECIFIED Ponnu, Jathish UNSPECIFIED orcid.org/0000-0002-3276-7068 UNSPECIFIED Hoecker, Ute UNSPECIFIED orcid.org/0000-0002-5636-9777 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-196685
DOI:	10.1104/pp.17.01207
Journal or Publication Title:	Plant Physiol.
Volume:	176
Number:	2
Page Range:	S. 1327 - 1341
Date:	2018
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-2548
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS-THALIANA; LOCUS-T; MEDIATED DEGRADATION; PROTEIN INTERACTIONS; MOLYBDENUM COFACTOR; CONSTANS PROTEIN; GENE-EXPRESSION; LIGHT; INTERACTS; GROWTH Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19668