Universität zu Köln

Hoffmann, Gesine, Samel-Pommerencke, Anke, Weber, Jan, Cuomo, Alessandro, Bonaldi, Tiziana ORCID: 0000-0003-3556-1265 and Ehrenhofer-Murray, Ann E. (2018). A role for CENP-A/Cse4 phosphorylation on serine 33 in deposition at the centromere. FEMS Yeast Res., 18 (1). OXFORD: OXFORD UNIV PRESS. ISSN 1567-1364

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Abstract

Centromeres are the sites of assembly of the kinetochore, which connect the chromatids to the microtubules for sister chromatid segregation during cell division. Centromeres are characterized by the presence of the histone H3 variant CENP-A (termed Cse4 in Saccharomyces cerevisiae). Here, we investigated the function of serine 33 phosphorylation of Cse4 (Cse4-S33ph) in S. cerevisiae, which lies within the essential N-terminal domain (END) of the extended Cse4 N-terminus. Significantly, we identified histone H4-K5, 8, 12R to cause a temperature-sensitive growth defect with mutations in Cse4-S33 and sensitivity to nocodazole and hydroxyurea. Furthermore, the absence of Cse4-S33ph reduced the levels of Cse4 at centromeric sequences, suggesting that Cse4 deposition is defective in the absence of S33 phosphorylation. We furthermore identified synthetic genetic interactions with histone H2A-E57A and H2A-L66A, which both cause a reduced interaction with the histone chaperone FACT and reduced H2A/H2B levels in chromatin, again supporting the notion that a combined defect of H2A/H2B and Cse4 deposition causes centromeric defects. Altogether, our data highlight the importance of correct histone deposition in building a functional centromeric nucleosome and suggests a role for Cse4-S33ph in this process.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hoffmann, Gesine UNSPECIFIED UNSPECIFIED UNSPECIFIED Samel-Pommerencke, Anke UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Cuomo, Alessandro UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonaldi, Tiziana UNSPECIFIED orcid.org/0000-0003-3556-1265 UNSPECIFIED Ehrenhofer-Murray, Ann E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-196921
DOI:	10.1093/femsyr/fox094
Journal or Publication Title:	FEMS Yeast Res.
Volume:	18
Number:	1
Date:	2018
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1567-1364
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CENP-A NUCLEOSOMES; HISTONE H3 VARIANT; E3 UBIQUITIN LIGASE; GLOBAL ANALYSIS; KINETOCHORE RECRUITMENT; GENETIC-ANALYSIS; STRUCTURAL BASIS; CORE HISTONES; YEAST; PROTEIN Multiple languages Biotechnology & Applied Microbiology; Microbiology; Mycology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19692