Universität zu Köln

Burghartz, Marc, Lennartz, Simon, Schweinlin, Matthias, Hagen, Rudolf, Kleinsasser, Norbert, Hackenberg, Stephan, Steussloff, Gudrun, Scherzad, Agmal, Radeloff, Kathrin, Ginzkey, Christian, Walles, Heike ORCID: 0000-0002-1512-7448 and Metzger, Marco (2018). Development of Human Salivary Gland-Like Tissue In Vitro. Tissue Eng. Part A, 24 (3-4). S. 301 - 310. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1937-335X

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Abstract

The loss of salivary gland function caused by radiation therapy of the head and neck is a serious condition and it affects a patient's quality of life. The current lack of effective therapies demands new options to be explored. This study tested whether human salivary gland epithelial cells (SGECs) could be successfully cultured on a decellularized porcine gut matrix (SIS-muc) in both mono- and coculture with microvascular endothelial cells (mvECs). By performing immunofluorescence imaging, transmission as well as scanning electron microscopy (SEM), quantitative polymerase chain reaction (qPCR), and an amylase enzyme assay, it was investigated as to what extent the three-dimensional (3D)-cultured cells could maintain their molecular differentiation and the production of working -amylase (-AMY) compared with two-dimensional (2D) culture. In both 3D mono- and coculture, SGECs were successfully cultured and formed acinar-like structures. Those findings were confirmed by SEM imaging. Immunofluorescence imaging revealed that 3D-cultured cells expressed -AMY, Claudin-1 (CL-1), and water channel protein aquaporin-5 (AQP-5). Two-dimensional-cultured cells only were positive for -AMY. Real time (RT)-qPCR analysis showed that -AMY relative gene expression was higher in both 3D mono- and coculture than in 2D culture. In -AMY enzyme assay, cocultured SGECs showed about 25 times increased enzyme activity compared with 2D-cultured cells. In conclusion, the SIS-muc combined with endothelial coculture seems a suitable culture setting for the tissue engineering of functional human salivary gland tissue.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Burghartz, Marc UNSPECIFIED UNSPECIFIED UNSPECIFIED Lennartz, Simon UNSPECIFIED UNSPECIFIED UNSPECIFIED Schweinlin, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Hagen, Rudolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Kleinsasser, Norbert UNSPECIFIED UNSPECIFIED UNSPECIFIED Hackenberg, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Steussloff, Gudrun UNSPECIFIED UNSPECIFIED UNSPECIFIED Scherzad, Agmal UNSPECIFIED UNSPECIFIED UNSPECIFIED Radeloff, Kathrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Ginzkey, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Walles, Heike UNSPECIFIED orcid.org/0000-0002-1512-7448 UNSPECIFIED Metzger, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-198056
DOI:	10.1089/ten.tea.2016.0466
Journal or Publication Title:	Tissue Eng. Part A
Volume:	24
Number:	3-4
Page Range:	S. 301 - 310
Date:	2018
Publisher:	MARY ANN LIEBERT, INC
Place of Publication:	NEW ROCHELLE
ISSN:	1937-335X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SMALL-INTESTINAL SUBMUCOSA; INTENSITY-MODULATED RADIOTHERAPY; HUMAN SUBMANDIBULAR-GLAND; 2-STAGE AUTOTRANSPLANTATION; LUMEN FORMATION; ACINAR-CELLS; XEROSTOMIA; SCAFFOLDS; HEAD; ORGANIZATION Multiple languages Cell & Tissue Engineering; Cell Biology; Engineering, Biomedical; Materials Science, Biomaterials Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19805