Universität zu Köln

Springer, Jan, White, P. Lewis, Kessel, Johanna, Wieters, Imke, Teschner, Daniel ORCID: 0000-0001-7351-8060, Korczynski, Daniel, Liebregts, Tobias, Cornely, Oliver A., Schwartz, Stefan ORCID: 0000-0001-8833-5793, Elgeti, Thomas, Meintker, Lisa, Krause, Stefan W., Posso, Raquel B., Heinz, Werner J., Fuhrmann, Sandra, Vehreschild, Joerg Janne, Einsele, Hermann, Rickerts, Volker and Loeffler, Juergen (2018). A Comparison of Aspergillus and Mucorales PCR Testing of Different Bronchoalveolar Lavage Fluid Fractions from Patients with Suspected Invasive Pulmonary Fungal Disease. J. Clin. Microbiol., 56 (2). WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-660X

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Abstract

In patients with hematological malignancies, bronchoalveolar lavage fluid (BALF) specimens are commonly used for the diagnosis of mold infections. However, it is not clear whether the cell pellet (P) or the supernatant fraction (S) of the BALF specimen is optimal for molecular diagnostic testing. Thus, 99 BALF specimens were collected from 96 hematology patients with or without allogeneic hematopoietic stem cell transplant. The cell pellets and supernatants were processed alone and in combination (S/P) for testing by two fungus-specific real-time PCR assays compliant with international recommendations. The results achieved with S/P were revealed to be superior in comparison to those achieved with S and P alone, with the use of each single fraction showing a reduced sensitivity for the detection of Aspergillus DNA (82% and 43% for S and P, respectively). In 57% of the samples, testing of the combination of S and P generated a lower quantification cycle value than testing of S or P alone. Molds would have been missed in 5 and 16 out of 28 samples if only S or P, respectively, was analyzed. No sample was positive by testing of S or P only. Similar results were obtained for the detection of Mucorales DNA in BALF specimens (reduced sensitivity of 67% and 50% for S and P, respectively). Study patients were categorized according to the current European Organization for the Research and Treatment of Cancer/Mycoses Study Group classification for invasive fungal disease (IFD), revealing that 35 patients had proven/probable IFD (36%), 47 patients had possible IFD (49%), and 14 patients had undetermined IFD (15%).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Springer, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED White, P. Lewis UNSPECIFIED UNSPECIFIED UNSPECIFIED Kessel, Johanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Wieters, Imke UNSPECIFIED UNSPECIFIED UNSPECIFIED Teschner, Daniel UNSPECIFIED orcid.org/0000-0001-7351-8060 UNSPECIFIED Korczynski, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Liebregts, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Cornely, Oliver A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwartz, Stefan UNSPECIFIED orcid.org/0000-0001-8833-5793 UNSPECIFIED Elgeti, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Meintker, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Krause, Stefan W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Posso, Raquel B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Heinz, Werner J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fuhrmann, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Vehreschild, Joerg Janne UNSPECIFIED UNSPECIFIED UNSPECIFIED Einsele, Hermann UNSPECIFIED UNSPECIFIED UNSPECIFIED Rickerts, Volker UNSPECIFIED UNSPECIFIED UNSPECIFIED Loeffler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-198153
DOI:	10.1128/JCM.01655-17
Journal or Publication Title:	J. Clin. Microbiol.
Volume:	56
Number:	2
Date:	2018
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-660X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HIGH-RISK PATIENTS; REAL-TIME PCR; GALACTOMANNAN; MALIGNANCIES; INFECTIONS; DIAGNOSIS; BURDEN; PLASMA Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19815