Rawstron, Andy C., Kreuzer, Karl-Anton, Soosapilla, Asha, Spacek, Martin ORCID: 0000-0001-5250-8218, Stehlikova, Olga, Gambell, Peter, McIver-Brown, Neil, Villamor, Neus, Psarra, Katherina, Arroz, Maria, Milani, Raffaella, de la Serna, Javier, Teresa Cedena, M., Jaksic, Ozren, Nomdedeu, Josep ORCID: 0000-0003-3399-346X, Moreno, Carol, Rigolin, Gian Matteo, Cuneo, Antonio, Johansen, Preben, Johnsen, Hans E., Rosenquist, Richard, Niemann, Carsten Utoft, Kern, Wolfgang, Westerman, David, Trneny, Marek, Mulligan, Stephen, Doubek, Michael, Pospisilova, Sarka, Hillmen, Peter, Oscier, David, Hallek, Michael, Ghia, Paolo ORCID: 0000-0003-3750-7342 and Montserrat, Emili (2018). Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project. Cytom. Part B-Clin. Cytom., 94 (1). S. 121 - 129. HOBOKEN: WILEY. ISSN 1552-4957

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Abstract

The diagnostic criteria for CLL rely on morphology and immunophenotype. Current approaches have limitations affecting reproducibility and there is no consensus on the role of new markers. The aim of this project was to identify reproducible criteria and consensus on markers recommended for the diagnosis of CLL. ERIC/ESCCA members classified 14 of 35 potential markers as required or recommended for CLL diagnosis, consensus being defined as >75% and >50% agreement, respectively. An approach to validate required markers using normal peripheral blood was developed. Responses were received from 150 participants with a diagnostic workload >20 CLL cases per week in 23/150 (15%), 5-20 in 82/150 (55%), and <5 cases per week in 45/150 (30%). The consensus for required diagnostic markers included: CD19, CD5, CD20, CD23, Kappa, and Lambda. Recommended markers potentially useful for differential diagnosis were: CD43, CD79b, CD81, CD200, CD10, and ROR1. Reproducible criteria for component reagents were assessed retrospectively in 14,643 cases from 13 different centers and showed >97% concordance with current approaches. A pilot study to validate staining quality was completed in 11 centers. Markers considered as required for the diagnosis of CLL by the participants in this study (CD19, CD5, CD20, CD23, Kappa, and Lambda) are consistent with current diagnostic criteria and practice. Importantly, a reproducible approach to validate and apply these markers in individual laboratories has been identified. Finally, a consensus recommended panel of markers to refine diagnosis in borderline cases (CD43, CD79b, CD81, CD200, CD10, and ROR1) has been defined and will be prospectively evaluated. (c) 2017 International Clinical Cytometry Society

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Rawstron, Andy C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kreuzer, Karl-AntonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Soosapilla, AshaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Spacek, MartinUNSPECIFIEDorcid.org/0000-0001-5250-8218UNSPECIFIED
Stehlikova, OlgaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gambell, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
McIver-Brown, NeilUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Villamor, NeusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Psarra, KatherinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Arroz, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Milani, RaffaellaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
de la Serna, JavierUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Teresa Cedena, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jaksic, OzrenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nomdedeu, JosepUNSPECIFIEDorcid.org/0000-0003-3399-346XUNSPECIFIED
Moreno, CarolUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rigolin, Gian MatteoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cuneo, AntonioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Johansen, PrebenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Johnsen, Hans E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rosenquist, RichardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Niemann, Carsten UtoftUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kern, WolfgangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Westerman, DavidUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Trneny, MarekUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mulligan, StephenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doubek, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pospisilova, SarkaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hillmen, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Oscier, DavidUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hallek, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ghia, PaoloUNSPECIFIEDorcid.org/0000-0003-3750-7342UNSPECIFIED
Montserrat, EmiliUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-200289
DOI: 10.1002/cyto.b.21595
Journal or Publication Title: Cytom. Part B-Clin. Cytom.
Volume: 94
Number: 1
Page Range: S. 121 - 129
Date: 2018
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1552-4957
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
RECEPTOR TYROSINE KINASE; SCORING SYSTEM; EXPRESSION; ROR1; GUIDELINES; LYMPHOMA; CD20; CD38Multiple languages
Medical Laboratory Technology; PathologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/20028

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