Universität zu Köln

von Brandenstein, Melanie, Schlosser, Monika, Herden, Jan, Heidenreich, Axel, Stoerkel, Stefan and Fries, Jochen W. U. (2018). MicroRNAs as Urinary Biomarker for Oncocytoma. Dis. Markers. LONDON: HINDAWI LTD. ISSN 1875-8630

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Abstract

The identification of benign renal oncocytoma, its differentiation from malignant renal tumors, and their eosinophilic variants are a continuous challenge, influencing preoperative planning and being an unnecessary stress factor for patients. Regressive changes enhance the diagnostic dilemma, making evaluations by frozen sections or by immunohistology (on biopsies) unreliable. MicroRNAs (miRs) have been proposed as novel biomarkers to differentiate renal tumor subtypes. However, their value as a diagnostic biomarker of oncocytoma in urines based on mechanisms known in oncocytomas has not been exploited. We used urines from patients with renal tumors (oncocytoma, renal cell carcinoma: clear cell, papillary, chromophobe) and with other urogenital lesions. miRs were extracted and detected via qRT-PCR, the respective tumors analyzed by immunohistology. We found isocitrate dehydrogenase 2 upregulated in oncocytoma and oncocytic chromophobe carcinoma, indicating an increased Krebs cycle metabolism. Since we had shown that all renal tumors are stimulated by endothelin-1, we analyzed miRs preidentified by microarray after endothelin-1 stimulation of renal epithelial cells. Four miRs are proposed as presurgical urinary biomarkers due to their known regulatory mechanism in oncocytoma: miR-498 (formation of the oncocytoma-specific slice-form of vimentin, Vim3), miR-183 (associated with increased CO2 levels), miR-205, and miR-31 (signaling through downregulation of PKC epsilon, shown previously).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code von Brandenstein, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlosser, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Herden, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Heidenreich, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Stoerkel, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Fries, Jochen W. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-200297
DOI:	10.1155/2018/6979073
Journal or Publication Title:	Dis. Markers
Date:	2018
Publisher:	HINDAWI LTD
Place of Publication:	LONDON
ISSN:	1875-8630
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RENAL-CELL CARCINOMA; EPITHELIAL-MESENCHYMAL TRANSITION; EXPRESSION LEVELS; CANDIDATE MIRNAS; DOWN-REGULATION; CANCER; TUMOR; MIR-205; PROGNOSIS; INVASION Multiple languages Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental; Pathology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20029