Universität zu Köln

Sapp, Melanie ORCID: 0000-0002-0817-5425, Ploch, Sebastian ORCID: 0000-0002-7129-4523, Fiore-Donno, Anna M., Bonkowski, Michael ORCID: 0000-0003-2656-1183 and Rose, Laura E. (2018). Protists are an integral part of the Arabidopsis thaliana microbiome. Environ. Microbiol., 20 (1). S. 30 - 44. HOBOKEN: WILEY. ISSN 1462-2920

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Abstract

Although protists occupy a vast range of habitats and are known to interact with plants among other things via disease suppression, competition or growth stimulation, their contributions to the phytobiome' are not well described. To contribute to a more comprehensive picture of the plant holobiont, we examined cercozoan and oomycete taxa living in association with the model plant Arabidopsis thaliana grown in two different soils. Soil, roots, leaves and wooden toothpicks were analysed before and after surface sterilization. Cercozoa were identified using 18S rRNA gene metabarcoding, whereas the Internal Transcribed Spacer 1 was used to determine oomycetes. Subsequent analyses revealed strong spatial structuring of protist communities between compartments, although oomycetes appeared more specialized than Cercozoa. With regards to oomycetes, only members of the Peronosporales and taxa belonging to the genus Globisporangium were identified as shared members of the A. thaliana microbiome. This also applied to cercozoan taxa belonging to the Glissomonadida and Cercomonadida. We identified a strong influence by edaphic factors on the rhizosphere, but not for the phyllosphere. Distinct differences of Cercozoa found preferably in wood or fresh plant material imply specific niche adaptations. Our results highlight the importance of micro-eukaryotes for the plant holobiont.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sapp, Melanie UNSPECIFIED orcid.org/0000-0002-0817-5425 UNSPECIFIED Ploch, Sebastian UNSPECIFIED orcid.org/0000-0002-7129-4523 UNSPECIFIED Fiore-Donno, Anna M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonkowski, Michael UNSPECIFIED orcid.org/0000-0003-2656-1183 UNSPECIFIED Rose, Laura E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-200346
DOI:	10.1111/1462-2920.13941
Journal or Publication Title:	Environ. Microbiol.
Volume:	20
Number:	1
Page Range:	S. 30 - 44
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1462-2920
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Geosciences
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BACTERIAL COMMUNITY COMPOSITION; RHIZARIA CERCOZOA; RHIZOSPHERE MICROBIOME; OOMYCETE COMMUNITIES; NAKED FILOSE; GEN. NOV; SOIL; DIVERSITY; PROTOZOA; GLISSOMONADIDA Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20034