Universität zu Köln

Tempfer, Herbert, Kaser-Eichberger, Alexandra, Lehner, Christine, Gehwolf, Renate, Korntner, Stefanie ORCID: 0000-0002-5937-9938, Kunkel, Nadja, Wagner, Andrea, Gruetz, Moritz, Heindl, Ludwig M., Schroedl, Falk and Traweger, Andreas (2018). Bevacizumab Improves Achilles Tendon Repair in a Rat Model. Cell. Physiol. Biochem., 46 (3). S. 1148 - 1159. BASEL: KARGER. ISSN 1421-9778

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Abstract

Background/Aims: Effective wound-healing generally requires efficient re-vascularization after injury, ensuring sufficient supply with oxygen, nutrients, and various cell populations. While this applies to most tissues, tendons are mostly avascular in nature and harbor relatively few cells, probably contributing to their poor regenerative capacity. Considering the minimal vascularization of healthy tendons, we hypothesize that controlling angiogenesis in early tendon healing is beneficial for repair tissue quality and function. Methods: To address this hypothesis, Bevacizumab, a monoclonal antibody blocking VEGF-A signaling, was locally injected into the defect area of a complete tenotomy in rat Achilles tendon. At 28 days post-surgery, the defect region was investigated using immunohistochemistry against vascular and lymphatic epitopes. Polarization microscopy and biomechanical testing was used to determine tendon integrity and gait analysis for functional testing in treated vs non-treated animals. Results: Angiogenesis was found to be significantly reduced in the Bevacizumab treated repair tissue, accompanied by significantly reduced cross sectional area, improved matrix organization, increased stiffness and Young's modulus, maximum load and stress. Further, we observed an improved gait pattern when compared to the vehicle injected control group. Conclusion: Based on the results of this study we propose that reducing angiogenesis after tendon injury can improve tendon repair, potentially representing a novel treatment-option. (C) 2018 The Author(s) Published by S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tempfer, Herbert UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaser-Eichberger, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Lehner, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Gehwolf, Renate UNSPECIFIED UNSPECIFIED UNSPECIFIED Korntner, Stefanie UNSPECIFIED orcid.org/0000-0002-5937-9938 UNSPECIFIED Kunkel, Nadja UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagner, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Gruetz, Moritz UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, Ludwig M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroedl, Falk UNSPECIFIED UNSPECIFIED UNSPECIFIED Traweger, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-202955
DOI:	10.1159/000489057
Journal or Publication Title:	Cell. Physiol. Biochem.
Volume:	46
Number:	3
Page Range:	S. 1148 - 1159
Date:	2018
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1421-9778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOTHELIAL GROWTH-FACTOR; ROTATOR CUFF REPAIR; MONOCLONAL-ANTIBODIES; SCAR FORMATION; IN-VIVO; THERAPY; CELLS; ANGIOGENESIS; TENDINOPATHY; CANCER Multiple languages Cell Biology; Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20295