Universität zu Köln

Hong, Xian, Yuan, Yin, Sun, Xiaoxi, Zhou, Meiling, Guo, Guangyu, Zhang, Quan, Hescheler, Juergen and Xi, Jiaoya (2018). Skeletal Extracellular Matrix Supports Cardiac Differentiation of Embryonic Stem Cells: a Potential Scaffold for Engineered Cardiac Tissue. Cell. Physiol. Biochem., 45 (1). S. 319 - 332. BASEL: KARGER. ISSN 1421-9778

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Abstract

Background/Aims: Decellularized cardiac extracellular matrix (cECM) has been widely considered as an attractive scaffold for engineered cardiac tissue (ECT), however, its application is limited by immunogenicity and shortage of organ donation. Skeletal ECM (sECM) is readily available and shows similarities with cECM. Here we hypothesized that sECM might be an alternative scaffold for ECT strategies. Methods: Murine ventricular tissue and anterior tibial muscles were sectioned into 300 mm-thick, and then cECM and sECM were acquired by pretreatment/SDS/TritonX-100 three-step-method. Acellularity and morphological properties of ECM was assessed. SECM was recellularized with murine embryonic stem cells (mESCs) or mESC-derived cardiomyocytes (mESC-CMs), and was further studied by biocompatibility assessment, immunofluorescent staining, quantitative real-time PCR and electrophysiological experiment. Results: The relative residual contents of DNA, protein and RNA of sECM were comparable with cECM. The morphological properties and microstructure of sECM were similar to cECM. SECM supported mESCs to adhere, survive, proliferate and differentiate into functional cardiac microtissue with both electrical stimulated response and normal adrenergic response. Purified mESC-CMs also could adhere, survive, proliferate and form a sECM-based ECT with synchronized contraction within 6 days of recellularization. Conclusion: ECMs from murine skeletal muscle support survival and cardiac differentiation of mESCs, and are suitable to produce functional ECT patch. This study highlights the potential of patient specific of sECM to replace cECM for bioengineering ECT. (C) 2018 The Author(s) Published by S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hong, Xian UNSPECIFIED UNSPECIFIED UNSPECIFIED Yuan, Yin UNSPECIFIED UNSPECIFIED UNSPECIFIED Sun, Xiaoxi UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhou, Meiling UNSPECIFIED UNSPECIFIED UNSPECIFIED Guo, Guangyu UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Quan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Xi, Jiaoya UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-204087
DOI:	10.1159/000486813
Journal or Publication Title:	Cell. Physiol. Biochem.
Volume:	45
Number:	1
Page Range:	S. 319 - 332
Date:	2018
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1421-9778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NITRIC-OXIDE; INFARCTED MYOCARDIUM; BIOLOGIC SCAFFOLD; IN-VITRO; HEART; MUSCLE; MURINE; REPAIR; CARDIOMYOCYTES; CONTRACTILE Multiple languages Cell Biology; Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20408