Mesarich, Carl H., Okmen, Bilal, Rovenich, Hanna, Griffiths, Scott A., Wang, Changchun, Jashni, Mansoor Karimi, Mihajlovski, Aleksandar, Collemare, Jerome ORCID: 0000-0001-8152-6642, Hunziker, Lukas ORCID: 0000-0002-4678-6518, Deng, Cecilia H., van der Burgt, Ate, Beenen, Henriek G., Templeton, Matthew D. ORCID: 0000-0003-0192-9041, Bradshaw, Rosie E. and de Wit, Pierre J. G. M. (2018). Specific Hypersensitive Response-Associated Recognition of New Apoplastic Effectors from Cladosporium fulvum in Wild Tomato. Mol. Plant-Microbe Interact., 31 (1). S. 145 - 163. ST PAUL: AMER PHYTOPATHOLOGICAL SOC. ISSN 1943-7706

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Abstract

Tomato leaf mold disease is caused by the biotrophic fungus Cladosporium fulvum. During infection, C. fulvum produces extracellular small secreted protein (SSP) effectors that function to promote colonization of the leaf apoplast. Resistance to the disease is governed by Cf immune receptor genes that encode receptor-like proteins (RLPs). These RLPs recognize specific SSP effectors to initiate a hypersensitive response (HR) that renders the pathogen avirulent. C. fulvum strains capable of overcoming one or more of all cloned Cf genes have now emerged. To combat these strains, new Cf genes are required. An effectoromics approach was employed to identify wild tomato accessions carrying new Cf genes. Proteomics and transcriptome sequencing were first used to identify 70 apoplastic in planta-induced C. fulvum SSPs. Based on sequence homology, 61 of these SSPs were novel or lacked known functional domains. Seven, however, had predicted structural homology to antimicrobial proteins, suggesting a possible role in mediating antagonistic microbe-microbe interactions in planta. Wild tomato accessions were then screened for HR-associated recognition of 41 SSPs, using the Potato virus X-based transient expression system. Nine SSPs were recognized by one or more accessions, suggesting that these plants carry new Cf genes available for incorporation into cultivated tomato.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Mesarich, Carl H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Okmen, BilalUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rovenich, HannaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Griffiths, Scott A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wang, ChangchunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jashni, Mansoor KarimiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mihajlovski, AleksandarUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Collemare, JeromeUNSPECIFIEDorcid.org/0000-0001-8152-6642UNSPECIFIED
Hunziker, LukasUNSPECIFIEDorcid.org/0000-0002-4678-6518UNSPECIFIED
Deng, Cecilia H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van der Burgt, AteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Beenen, Henriek G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Templeton, Matthew D.UNSPECIFIEDorcid.org/0000-0003-0192-9041UNSPECIFIED
Bradshaw, Rosie E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
de Wit, Pierre J. G. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-204957
DOI: 10.1094/MPMI-05-17-0114-FI
Journal or Publication Title: Mol. Plant-Microbe Interact.
Volume: 31
Number: 1
Page Range: S. 145 - 163
Date: 2018
Publisher: AMER PHYTOPATHOLOGICAL SOC
Place of Publication: ST PAUL
ISSN: 1943-7706
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CF-2-DEPENDENT DISEASE RESISTANCE; PATHOGEN DOTHISTROMA-SEPTOSPORUM; PROTEIN FOLD RECOGNITION; CERATO-PLATANIN PROTEIN; LEUCINE-RICH REPEATS; VIRUS-ENCODED TOXIN; YEAST KILLER TOXIN; BETA-BARREL FOLD; F-SP PLATANI; USTILAGO-MAYDISMultiple languages
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/20495

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