Universität zu Köln

Robbins, Chanz ORCID: 0000-0002-8044-4941, Thiergart, Thorsten, Hacquard, Stephane, Garrido-Oter, Ruben, Gans, Wolfgang, Peiter, Edgar ORCID: 0000-0002-9104-3238, Schulze-Lefert, Paul and Spaepen, Stijn ORCID: 0000-0001-5465-8287 (2018). Root-Associated Bacterial and Fungal Community Profiles of Arabidopsis thaliana Are Robust Across Contrasting Soil P Levels. Phytobiomes J., 2 (1). S. 24 - 35. ST PAUL: AMER PHYTOPATHOLOGICAL SOC. ISSN 2471-2906

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Abstract

Plant survival depends on the ability of roots to sense and acquire nutrients in soils, which harbor a rich diversity of microbes. A subset of this microcosm interacts with plant roots and collectively forms root-associated microbial communities, termed the root microbiota. Under phosphorus-limiting conditions, some plants can engage in mutualistic interactions, for example with arbuscular mycorrhizal fungi. Here, we describe how Arabidopsis thaliana, which lacks the genetic capacity for establishing the aforementioned symbiosis, interacts with soil-resident bacteria and fungi in soil from a long-term phosphorus fertilization trial. Long-term, contrasting fertilization regimes resulted in an similar to 6-fold and similar to 2.4-fold disparity in bioavailable and total phosphorous, respectively, which may explain differences in biomass of A. thaliana plants. Sequencing of marker genes enabled us to characterize bacterial and fungal communities present in the bulk soil, rhizosphere, and root compartments. Phosphorus had little effect on alpha- or beta-diversity indices, but more strongly influences bacterial and fungal community shifts in plant-associated compartments compared with bulk soil. The significant impact of soil P abundance could only be resolved at operational taxonomic unit level, and these subtle differences are more pronounced in the root compartment. We conclude that despite decades of different fertilization, both bacterial and fungal soil communities remained unexpectedly stable in soils tested, suggesting that the soil biota is resilient over time to nutrient supplementation. Conversely, low-abundance, root-associated microbes, which collectively represent 2 to 3% of the relative abundance of bacteria and fungi in the roots, exhibited a subtle, yet significant shift between the two soils.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Robbins, Chanz UNSPECIFIED orcid.org/0000-0002-8044-4941 UNSPECIFIED Thiergart, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Hacquard, Stephane UNSPECIFIED UNSPECIFIED UNSPECIFIED Garrido-Oter, Ruben UNSPECIFIED UNSPECIFIED UNSPECIFIED Gans, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED Peiter, Edgar UNSPECIFIED orcid.org/0000-0002-9104-3238 UNSPECIFIED Schulze-Lefert, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED Spaepen, Stijn UNSPECIFIED orcid.org/0000-0001-5465-8287 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-205057
DOI:	10.1094/PBIOMES-09-17-0042-R
Journal or Publication Title:	Phytobiomes J.
Volume:	2
Number:	1
Page Range:	S. 24 - 35
Date:	2018
Publisher:	AMER PHYTOPATHOLOGICAL SOC
Place of Publication:	ST PAUL
ISSN:	2471-2906
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PHOSPHORUS FERTILIZATION; MICROBIAL COMMUNITIES; BAYESIAN CLASSIFIER; PLANT; DIVERSITY; SEQUENCES; IDENTIFICATION; MECHANISMS; LIMITATION; DYNAMICS Multiple languages Plant Sciences; Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20505