Universität zu Köln

Waldera-Lupa, Daniel Michael, Etemad-Parishanzadeh, Omid ORCID: 0000-0003-3172-7512, Brocksieper, Mareike, Kirchgaessler, Nina, Seidel, Sabine, Kowalski, Thomas, Montesinos-Rongen, Manuel, Deckert, Martina, Schlegel, Uwe and Stuehler, Kai (2017). Proteomic changes in cerebrospinal fluid from primary central nervous system lymphoma patients are associated with protein ectodomain shedding. Oncotarget, 8 (66). S. 110118 - 110133. ORCHARD PARK: IMPACT JOURNALS LLC. ISSN 1949-2553

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Abstract

Primary central nervous system lymphomas (PCNSLs) are mature B-cell lymphomas confined to the central nervous system (CNS). Blood-brain barrier (BBB) dysfunction drastically alters the cerebrospinal fluid (CSF) proteome in PCNSL patients. To reveal the interaction of PCNSL tumors with CNS structures and the vasculature, we conducted a whole-proteome analysis of CSF from PCNSL patients (n = 17 at initial diagnosis) and tumor-free controls (n = 10) using label-free quantitative mass spectrometry. We identified 601 proteins in the CSF proteome using a one-step approach without further prefractionation, and quantified 438 proteins in detail using the Hi-N method. An immunoassay revealed that 70% of the patients in our unselected PCNSL patient cohort had BBB dysfunction. Correlation analysis indicated that 127 (30%) of the quantified proteins were likely increased in PCSNL patients due to BBB dysfunction. After the exclusion of these proteins, 66 were found to differ in abundance (fold-change > 2.0, p < 0.05) between PCNSL and control CSF proteomes, and most of those were associated with the CNS. These data also provide the first evidence that proteomic changes in CSF from PCNSL patients are mainly associated with protein ectodomain shedding, and that shedding of human leukocyte antigen class 2 proteins is a mechanism of tumor-cell immune evasion.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Waldera-Lupa, Daniel Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Etemad-Parishanzadeh, Omid UNSPECIFIED orcid.org/0000-0003-3172-7512 UNSPECIFIED Brocksieper, Mareike UNSPECIFIED UNSPECIFIED UNSPECIFIED Kirchgaessler, Nina UNSPECIFIED UNSPECIFIED UNSPECIFIED Seidel, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Kowalski, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Montesinos-Rongen, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Deckert, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlegel, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Stuehler, Kai UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-207846
DOI:	10.18632/oncotarget.22654
Journal or Publication Title:	Oncotarget
Volume:	8
Number:	66
Page Range:	S. 110118 - 110133
Date:	2017
Publisher:	IMPACT JOURNALS LLC
Place of Publication:	ORCHARD PARK
ISSN:	1949-2553
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CSF SOLUBLE CD27; CD163; EXPRESSION; DIAGNOSIS; BLOOD; GENE; INTERLEUKIN-10; IDENTIFICATION; ANNOTATION; BIOMARKER Multiple languages Oncology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20784