Universität zu Köln

Wewer, Vera ORCID: 0000-0002-0199-5228, Makepeace, Benjamin L., Tanya, Vincent N., Peisker, Helga, Pfarr, Kenneth ORCID: 0000-0003-3096-2465, Hoerauf, Achim and Doermann, Peter (2017). Lipid profiling of the filarial nematodes Onchocerca volvulus, Onchocerca ochengi and Litomosoides sigmodontis reveals the accumulation of nematode-specific ether phospholipids in the host. Int. J. Parasit., 47 (14). S. 903 - 913. OXFORD: ELSEVIER SCI LTD. ISSN 1879-0135

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Abstract

Onchocerciasis, a neglected tropical disease prevalent in western and central Africa, is a major health problem and has been targeted for elimination. The causative agent for this disease is the human parasite Onchocerca volvulus. Onchocerca ochengi and Litomosoides sigmodontis, infectious agents of cattle and rodents, respectively, serve as model organisms to study filarial nematode infections. Biomarkers to determine infection without the use of painful skin biopsies and microscopic identification of larval worms are needed and their discovery is facilitated by an improved knowledge of parasite-specific metabolites. In addition to proteins and nucleic acids, lipids may be suitable candidates for filarial biomarkers that are currently underexplored. To fill this gap, we present the phospholipid profile of the filarial nematodes O. ochengi, O. volvulus and L. sigmodontis. Direct infusion quadrupole time-of flight (Q-TOF) mass spectrometry was employed to analyze the composition of phospholipids and their molecular species in the three nematode species. Analysis of the phospholipid profiles of plasma or serum of uninfected and infected hosts showed that nematode-specific phospholipids were below detection limits. However, several phospholipids, in particular ether lipids of phosphatidylethanolamine (PE), were abundant in O. ochengi worms and in bovine nodule fluid, suggesting that these phospholipids might be released from O. ochengi into the host, and could serve as potential biomarkers. (C) 2017 The Author(s). Published by Elsevier Ltd on behalf of Australian Society for Parasitology.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wewer, Vera UNSPECIFIED orcid.org/0000-0002-0199-5228 UNSPECIFIED Makepeace, Benjamin L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tanya, Vincent N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Peisker, Helga UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfarr, Kenneth UNSPECIFIED orcid.org/0000-0003-3096-2465 UNSPECIFIED Hoerauf, Achim UNSPECIFIED UNSPECIFIED UNSPECIFIED Doermann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-208824
DOI:	10.1016/j.ijpara.2017.06.001
Journal or Publication Title:	Int. J. Parasit.
Volume:	47
Number:	14
Page Range:	S. 903 - 913
Date:	2017
Publisher:	ELSEVIER SCI LTD
Place of Publication:	OXFORD
ISSN:	1879-0135
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BIOMARKER; PARASITE Multiple languages Parasitology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20882