Universität zu Köln

Rippin, Martin ORCID: 0000-0003-4362-0122, Becker, Burkhard ORCID: 0000-0002-7965-1396 and Holzinger, Andreas ORCID: 0000-0002-7745-3978 (2017). Enhanced Desiccation Tolerance in Mature Cultures of the Streptophytic Green Alga Zygnema circumcarinatum Revealed by Transcriptomics. Plant Cell Physiol., 58 (12). S. 2067 - 2085. OXFORD: OXFORD UNIV PRESS. ISSN 1471-9053

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Abstract

Desiccation tolerance is commonly regarded as one of the key features for the colonization of terrestrial habitats by green algae and the evolution of land plants. Extensive studies, focused mostly on physiology, have been carried out assessing the desiccation tolerance and resilience of the streptophytic genera Klebsormidium and Zygnema. Here we present transcriptomic analyses of Zygnema circumcarinatum exposed to desiccation stress. Cultures of Z. circumcarinatum grown in liquid medium or on agar plates were desiccated at similar to 86% relative air humidity until the effective quantum yield of PSII [Y(II)] ceased. In general, the response to dehydration was much more pronounced in Z. circumcarinatum cultured in liquid medium for 1 month compared with filaments grown on agar plates for 7 and 12 months. Culture on solid medium enables the alga to acclimate to dehydration much better and an increase in desiccation tolerance was clearly correlated to increased culture age. Moreover, gene expression analysis revealed that photosynthesis was strongly repressed upon desiccation treatment in the liquid culture while only minor effects were detected in filaments cultured on agar plates for 7 months. Otherwise, both samples showed induction of stress protection mechanisms such as reactive oxygen species scavenging (early light-induced proteins, glutathione metabolism) and DNA repair as well as the expression of chaperones and aquaporins. Additionally, Z. circumcarinatum cultured in liquid medium upregulated sucrose-synthesizing enzymes and strongly induced membrane modifications in response to desiccation stress. These results corroborate the previously described hardening and associated desiccation tolerance in Zygnema in response to seasonal fluctuations in water availability.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rippin, Martin UNSPECIFIED orcid.org/0000-0003-4362-0122 UNSPECIFIED Becker, Burkhard UNSPECIFIED orcid.org/0000-0002-7965-1396 UNSPECIFIED Holzinger, Andreas UNSPECIFIED orcid.org/0000-0002-7745-3978 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-210391
DOI:	10.1093/pcp/pcx136
Journal or Publication Title:	Plant Cell Physiol.
Volume:	58
Number:	12
Page Range:	S. 2067 - 2085
Date:	2017
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1471-9053
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HEAT-SHOCK PROTEINS; PLANT CRATEROSTIGMA-PLANTAGINEUM; GENE-EXPRESSION; DROUGHT STRESS; HIGH LIGHT; EARLY DIVERSIFICATION; LOW-TEMPERATURE; LEA PROTEIN; COLD STRESS; GENOME Multiple languages Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21039