Universität zu Köln

Schmitz, Jessica, Dittmar, Isabell C., Brockmann, Joern D., Schmidt, Marc, Huedig, Meike, Rossoni, Alessandro W. and Maurino, Veronica G. (2017). Defense against Reactive Carbonyl Species Involves at Least Three Subcellular Compartments Where Individual Components of the System Respond to Cellular Sugar Status. Plant Cell, 29 (12). S. 3234 - 3255. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-298X

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Abstract

Methylglyoxal (MGO) and glyoxal (GO) are toxic reactive carbonyl species generated as by-products of glycolysis. The pre-emption pathway for detoxification of these products, the glyoxalase (GLX) system, involves two consecutive reactions catalyzed by GLXI and GLXII. In Arabidopsis thaliana, the GLX system is encoded by three homologs of GLXI and three homologs of GLXII, from which several predicted GLXI and GLXII isoforms can be derived through alternative splicing. We identified the physiologically relevant splice forms using sequencing data and demonstrated that the resulting isoforms have different subcellular localizations. All three GLXI homologs are functional in vivo, as they complemented a yeast GLXI loss-of-function mutant. Efficient MGO and GO detoxification can be controlled by a switch in metal cofactor usage. MGO formation is closely connected to the flux through glycolysis and through the Calvin Benson cycle; accordingly, expression analysis indicated that GLXI is transcriptionally regulated by endogenous sugar levels. Analyses of Arabidopsis loss-of-function lines revealed that the elimination of toxic reactive carbonyl species during germination and seedling establishment depends on the activity of the cytosolic GLXI; 3 isoform. The Arabidopsis GLX system involves the cytosol, chloroplasts, and mitochondria, which harbor individual components that might be used at specific developmental stages and respond differentially to cellular sugar status.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schmitz, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Dittmar, Isabell C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmann, Joern D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Marc UNSPECIFIED UNSPECIFIED UNSPECIFIED Huedig, Meike UNSPECIFIED UNSPECIFIED UNSPECIFIED Rossoni, Alessandro W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maurino, Veronica G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-210415
DOI:	10.1105/tpc.17.00258
Journal or Publication Title:	Plant Cell
Volume:	29
Number:	12
Page Range:	S. 3234 - 3255
Date:	2017
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-298X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENOME-WIDE ANALYSIS; D-LACTATE DEHYDROGENASE; GLYOXALASE-I; ARABIDOPSIS-THALIANA; SUBSTRATE-SPECIFICITY; ESCHERICHIA-COLI; ENCODING GENES; METHYLGLYOXAL; PROTEIN; GLYCATION Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21041