Universität zu Köln

Lee, Hyun Ju, Bartsch, Deniz, Xiao, Cally ORCID: 0000-0001-7172-3175, Guerrero, Santiago ORCID: 0000-0003-3473-7214, Ahuja, Gaurav ORCID: 0000-0002-2837-9361, Schindler, Christina, Moresco, James J., Yates, John R., III, Gebauer, Fatima ORCID: 0000-0001-7563-0013, Bazzi, Hisham ORCID: 0000-0001-8388-4005, Dieterich, Christoph, Kurian, Leo ORCID: 0000-0001-7466-5169 and Vilchez, David ORCID: 0000-0002-0801-0743 (2017). A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. Nat. Commun., 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

Full text not available from this repository.

Abstract

While the transcriptional network of human embryonic stem cells (hESCs) has been extensively studied, relatively little is known about how post-transcriptional modulations determine hESC function. RNA-binding proteins play central roles in RNA regulation, including translation and turnover. Here we show that the RNA-binding protein CSDE1 (cold shock domain containing E1) is highly expressed in hESCs to maintain their undifferentiated state and prevent default neural fate. Notably, loss of CSDE1 accelerates neural differentiation and potentiates neurogenesis. Conversely, ectopic expression of CSDE1 impairs neural differentiation. We find that CSDE1 post-transcriptionally modulates core components of multiple regulatory nodes of hESC identity, neuroectoderm commitment and neurogenesis. Among these key pro-neural/neuronal factors, CSDE1 binds fatty acid binding protein 7 (FABP7) and vimentin (VIM) mRNAs, as well as transcripts involved in neuron projection development regulating their stability and translation. Thus, our results uncover CSDE1 as a central post-transcriptional regulator of hESC identity and neurogenesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lee, Hyun Ju UNSPECIFIED UNSPECIFIED UNSPECIFIED Bartsch, Deniz UNSPECIFIED UNSPECIFIED UNSPECIFIED Xiao, Cally UNSPECIFIED orcid.org/0000-0001-7172-3175 UNSPECIFIED Guerrero, Santiago UNSPECIFIED orcid.org/0000-0003-3473-7214 UNSPECIFIED Ahuja, Gaurav UNSPECIFIED orcid.org/0000-0002-2837-9361 UNSPECIFIED Schindler, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Moresco, James J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Yates, John R., III UNSPECIFIED UNSPECIFIED UNSPECIFIED Gebauer, Fatima UNSPECIFIED orcid.org/0000-0001-7563-0013 UNSPECIFIED Bazzi, Hisham UNSPECIFIED orcid.org/0000-0001-8388-4005 UNSPECIFIED Dieterich, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Kurian, Leo UNSPECIFIED orcid.org/0000-0001-7466-5169 UNSPECIFIED Vilchez, David UNSPECIFIED orcid.org/0000-0002-0801-0743 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-211301
DOI:	10.1038/s41467-017-01744-5
Journal or Publication Title:	Nat. Commun.
Volume:	8
Date:	2017
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RNA-BINDING PROTEIN; COLD-SHOCK DOMAINS; MESSENGER-RNA; N-RAS; IN-VITRO; TRANSLATIONAL REPRESSION; PRIMED PLURIPOTENCY; DOSAGE COMPENSATION; UNR TRANSLATION; NERVOUS-SYSTEM Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21130