Universität zu Köln

Zhang, Xiaoxiao, Liu, Weijia, Nagae, Takuya T., Takeuchi, Hidenori, Zhang, Heqiao ORCID: 0000-0003-0314-1573, Han, Zhifu, Higashiyama, Tetsuya ORCID: 0000-0002-7346-360X and Chai, Jijie (2017). Structural basis for receptor recognition of pollen tube attraction peptides. Nat. Commun., 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Transportation of the immobile sperms directed by pollen tubes to the ovule-enclosed female gametophytes is important for plant sexual reproduction. The defensin-like (DEFL) cysteine-rich peptides (CRPs) LUREs play an essential role in pollen tube attraction to the ovule, though their receptors still remain controversial. Here we provide several lines of biochemical evidence showing that the extracellular domain of the leucine-rich repeat receptor kinase (LRR-RK) PRK6 from Arabidopsis thaliana directly interacts with AtLURE1 peptides. Structural study reveals that a C-terminal loop of the LRR domain (AtPRK6(LRR)) is responsible for recognition of AtLURE1.2, mediated by a set of residues largely conserved among PRK6 homologs from Arabidopsis lyrata and Capsella rubella, supported by in vitro mutagenesis and semi-in-vivo pollen tube growth assays. Our study provides evidence showing that PRK6 functions as a receptor of the LURE peptides in A. thaliana and reveals a unique ligand recognition mechanism of LRR-RKs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zhang, Xiaoxiao UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Weijia UNSPECIFIED UNSPECIFIED UNSPECIFIED Nagae, Takuya T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Takeuchi, Hidenori UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Heqiao UNSPECIFIED orcid.org/0000-0003-0314-1573 UNSPECIFIED Han, Zhifu UNSPECIFIED UNSPECIFIED UNSPECIFIED Higashiyama, Tetsuya UNSPECIFIED orcid.org/0000-0002-7346-360X UNSPECIFIED Chai, Jijie UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-211496
DOI:	10.1038/s41467-017-01323-8
Journal or Publication Title:	Nat. Commun.
Volume:	8
Date:	2017
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TORENIA-FOURNIERI; CRYSTAL-STRUCTURE; SYNERGID CELL; GUIDANCE; GROWTH; ARABIDOPSIS; PERCEPTION; ACTIVATION; COMPLEX; IDENTIFICATION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21149