Pal, Kaushik, Heinsch, Angela, Berkessel, Albrecht ORCID: 0000-0003-0470-7428 and Koner, Apurba L.
(2017).
Differentiation of Folate-Receptor-Positive and -Negative Cells Using a Substrate-Mimicking Fluorescent Probe.
Chem.-Eur. J., 23 (60).
S. 15008 - 15012.
WEINHEIM:
WILEY-V C H VERLAG GMBH.
ISSN 1521-3765
Abstract
Diagnosis and therapy exploiting overexpressed receptors on the cell surface is one important strategy in medicine. Determination of the over expression level of a particular receptor is prerequisite for it to be of clinical use. Differentiation between FR-positive (FR=folate receptor) and -negative cells via fluorescence microscopy using a substrate mimetic fluorophore is presented in this work. The strategy adopted here is not the classical FA-conjugated (FA=folic acid) fluorescent probe but a small and environment-sensitive pterin-based (pterin is part of folate, i.e., vitaminB9) fluorescent probe. Electronically diverse pterin-based fluorescent probes have been designed and synthesized to understand the effect of the binding environment on the receptor-substrate interactions. By utilizing steady-state UV/Vis and fluorescence along with time-resolved fluorescence spectroscopy, the effects on the electronic and acid-base properties of the substrate were investigated. Evidently, one synthesized probe showed FA-mimicking behavior with strong binding interaction with FR.
Item Type: | Journal Article | ||||||||||||||||||||
Creators: |
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URN: | urn:nbn:de:hbz:38-213945 | ||||||||||||||||||||
DOI: | 10.1002/chem.201703305 | ||||||||||||||||||||
Journal or Publication Title: | Chem.-Eur. J. | ||||||||||||||||||||
Volume: | 23 | ||||||||||||||||||||
Number: | 60 | ||||||||||||||||||||
Page Range: | S. 15008 - 15012 | ||||||||||||||||||||
Date: | 2017 | ||||||||||||||||||||
Publisher: | WILEY-V C H VERLAG GMBH | ||||||||||||||||||||
Place of Publication: | WEINHEIM | ||||||||||||||||||||
ISSN: | 1521-3765 | ||||||||||||||||||||
Language: | English | ||||||||||||||||||||
Faculty: | Faculty of Mathematics and Natural Sciences | ||||||||||||||||||||
Divisions: | Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Organic Chemistry | ||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||
Uncontrolled Keywords: |
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Refereed: | Yes | ||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/21394 |
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