Universität zu Köln

Pal, Kaushik, Heinsch, Angela, Berkessel, Albrecht ORCID: 0000-0003-0470-7428 and Koner, Apurba L. (2017). Differentiation of Folate-Receptor-Positive and -Negative Cells Using a Substrate-Mimicking Fluorescent Probe. Chem.-Eur. J., 23 (60). S. 15008 - 15012. WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1521-3765

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Abstract

Diagnosis and therapy exploiting overexpressed receptors on the cell surface is one important strategy in medicine. Determination of the over expression level of a particular receptor is prerequisite for it to be of clinical use. Differentiation between FR-positive (FR=folate receptor) and -negative cells via fluorescence microscopy using a substrate mimetic fluorophore is presented in this work. The strategy adopted here is not the classical FA-conjugated (FA=folic acid) fluorescent probe but a small and environment-sensitive pterin-based (pterin is part of folate, i.e., vitaminB9) fluorescent probe. Electronically diverse pterin-based fluorescent probes have been designed and synthesized to understand the effect of the binding environment on the receptor-substrate interactions. By utilizing steady-state UV/Vis and fluorescence along with time-resolved fluorescence spectroscopy, the effects on the electronic and acid-base properties of the substrate were investigated. Evidently, one synthesized probe showed FA-mimicking behavior with strong binding interaction with FR.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pal, Kaushik UNSPECIFIED UNSPECIFIED UNSPECIFIED Heinsch, Angela UNSPECIFIED UNSPECIFIED UNSPECIFIED Berkessel, Albrecht UNSPECIFIED orcid.org/0000-0003-0470-7428 UNSPECIFIED Koner, Apurba L. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-213945
DOI:	10.1002/chem.201703305
Journal or Publication Title:	Chem.-Eur. J.
Volume:	23
Number:	60
Page Range:	S. 15008 - 15012
Date:	2017
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1521-3765
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Organic Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FOLIC-ACID; DIHYDROFOLATE-REDUCTASE; GOLD NANOPARTICLES; IN-VIVO; CANCER; DELIVERY; THERAPY; LIGAND; RECOGNITION; DIVERSITY Multiple languages Chemistry, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21394