Universität zu Köln

Firner, Sara, Zaucke, Frank, Michael, Joern, Dargel, Jens, Schiwy-Bochat, Karl-Heinz, Heilig, Juliane, Rothschild, Markus Alexander, Eysel, Peer, Brueggemann, Gert-Peter and Niehoff, Anja (2017). Extracellular Distribution of Collagen II and Perifibrillar Adapter Proteins in Healthy and Osteoarthritic Human Knee Joint Cartilage. J. Histochem. Cytochem., 65 (10). S. 593 - 607. LONDON: SAGE PUBLICATIONS LTD. ISSN 1551-5044

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Abstract

Perifibrillar adapter proteins, interconnecting collagen fibrils, and linking the collagen network with the aggrecan matrix seem to play a crucial role in the pathogenesis of osteoarthritis (OA). Therefore, we examined immunohistochemically the extracellular distribution of collagen II and the main perifibrillar adapter proteins-collagen IX, decorin, cartilage oligomeric matrix protein (COMP), and matrilin-3-in human samples of healthy (n= 4) and OA (n= 42) knee joint cartilage. Histopathology assessment was performed using an OA score. Staining patterns were evaluated in relation to the disease stage. The perifibrillar adapter proteins were uniformly distributed in the upper zones of healthy cartilage. In moderate OA (n= 8; score 14.3 +/- 4.7), all proteins analyzed were locally absent in the fibrillated area or the superficial and upper mid zone. In advanced OA (n= 20; score 18.9 +/- 5.3), they were uniformly distributed in these zones and accumulated pericellularly. Perifibrillar adapter proteins are important for the stabilization of the collagen network in the upper zones of healthy cartilage. Their degradation might be a critical event in early OA. In advanced OA, there are indications for an increased synthesis in an attempt to regenerate the lost tissue and to protect the remaining cartilage from further destruction.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Firner, Sara UNSPECIFIED UNSPECIFIED UNSPECIFIED Zaucke, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Michael, Joern UNSPECIFIED UNSPECIFIED UNSPECIFIED Dargel, Jens UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiwy-Bochat, Karl-Heinz UNSPECIFIED UNSPECIFIED UNSPECIFIED Heilig, Juliane UNSPECIFIED UNSPECIFIED UNSPECIFIED Rothschild, Markus Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Eysel, Peer UNSPECIFIED UNSPECIFIED UNSPECIFIED Brueggemann, Gert-Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Niehoff, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-216753
DOI:	10.1369/0022155417729154
Journal or Publication Title:	J. Histochem. Cytochem.
Volume:	65
Number:	10
Page Range:	S. 593 - 607
Date:	2017
Publisher:	SAGE PUBLICATIONS LTD
Place of Publication:	LONDON
ISSN:	1551-5044
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OLIGOMERIC MATRIX PROTEIN; MULTIPLE EPIPHYSEAL DYSPLASIA; HUMAN ARTICULAR-CARTILAGE; BONE-MINERAL DENSITY; IX COLLAGEN; PROTEOGLYCANS DECORIN; TISSUE DISTRIBUTION; VI MICROFIBRILS; SYNOVIAL-FLUID; CROSS-LINKING Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21675