Universität zu Köln

Almario, Juliana ORCID: 0000-0002-1475-7819, Jeena, Ganga, Wunder, Joerg, Langen, Gregor ORCID: 0000-0002-8321-1756, Zuccaro, Alga ORCID: 0000-0002-8026-0114, Coupland, George ORCID: 0000-0001-6988-4172 and Bucher, Marcel ORCID: 0000-0003-1680-9413 (2017). Root-associated fungal microbiota of nonmycorrhizal &ITArabis&IT &ITalpina &ITand its contribution to plant phosphorus nutrition. Proc. Natl. Acad. Sci. U. S. A., 114 (44). S. E9403 - 10. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

Most land plants live in association with arbuscular mycorrhizal (AM) fungi and rely on this symbiosis to scavenge phosphorus (P) from soil. The ability to establish this partnership has been lost in some plant lineages like the Brassicaceae, which raises the question of what alternative nutrition strategies such plants have to grow in P-impoverished soils. To understand the contribution of plant-micro biota interactions, we studied the root-associated fungal microbiome of Arabis alpina (Brassicaceae) with the hypothesis that some of its components can promote plant P acquisition. Using amplicon sequenc ing of the fungal internal transcribed spacer 2, we studied the root and rhizosphere fungal communities of A. alpina growing under natural and controlled conditions including low-P soils and identified a set of 15 fungal taxa consistently detected in its roots. This cohort included a Helotiales taxon exhibiting high abundance in roots of wild A. alpina growing in an extremely P-limited soil. Consequently, we isolated and subsequently reintroduced a specimen from this taxon into its native P-poor soil in which it improved plant growth and P uptake. The fungus exhibited mycorrhiza-like traits including colonization of the root endosphere and P transfer to the plant Genome analysis revealed a link between its endophytic lifestyle and the expansion of its repertoire of carbohydrate-active enzymes. We report the discovery of a plant- fungus interaction facilitating the growth of a nonmycorrhizal plant under native P-limited conditions, thus uncovering a previously underestimated role of root fungal microbiota in P cycling.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Almario, Juliana UNSPECIFIED orcid.org/0000-0002-1475-7819 UNSPECIFIED Jeena, Ganga UNSPECIFIED UNSPECIFIED UNSPECIFIED Wunder, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Langen, Gregor UNSPECIFIED orcid.org/0000-0002-8321-1756 UNSPECIFIED Zuccaro, Alga UNSPECIFIED orcid.org/0000-0002-8026-0114 UNSPECIFIED Coupland, George UNSPECIFIED orcid.org/0000-0001-6988-4172 UNSPECIFIED Bucher, Marcel UNSPECIFIED orcid.org/0000-0003-1680-9413 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-216979
DOI:	10.1073/pnas.1710455114
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	114
Number:	44
Page Range:	S. E9403 - 10
Date:	2017
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIS-ALPINA; RHIZOSPHERE MICROBIOME; SEQUENCE ALIGNMENT; ENDOPHYTIC FUNGUS; ARABIDOPSIS; SYMBIOSIS; ECOLOGY; BIOGEOGRAPHY; DIVERSITY; TRANSPORT Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21697