Universität zu Köln

Snwoldt, Juliane, Bosche, Bert, Meisel, Andreas ORCID: 0000-0001-7233-5342 and Mergenthaler, Philipp (2017). Neuronal Culture Microenvironments Determine Preferences in Bioenergetic Pathway Use. Front. Molec. Neurosci., 10. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1662-5099

Full text not available from this repository.

Abstract

In the brain, metabolic supply and demand is directly coupled to neuronal activation. Methods for culturing primary rodent brain cells have come of age and are geared toward sophisticated modeling of human brain physiology and pathology. However, the impact of the culture microenvironment on neuronal function is rarely considered. Therefore, we investigated the role of different neuronal culture supplements for neuronal survival and metabolic activity in a model of metabolic deprivation of neurons using oxygen deprivation, glucose deprivation, as well as live cell metabolic flux analysis. We demonstrate the impact of neuronal culture conditions on metabolic function and neuronal survival under conditions of metabolic stress. In particular, we find that the common neuronal cell culture supplement B27 protects neurons from cell death under hypoxic conditions and inhibits glycolysis. Furthermore, we present data that B27 as well as the alternative neuronal culture supplement N2 restrict neuronal glucose metabolism. On the contrary, we find that the more modern supplement GS21 promotes neuronal energy metabolism. Our data support the notion that careful control of the metabolic environment is an essential component in modeling brain function and the cellular and molecular pathophysiology of brain disease in culture.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Snwoldt, Juliane UNSPECIFIED UNSPECIFIED UNSPECIFIED Bosche, Bert UNSPECIFIED UNSPECIFIED UNSPECIFIED Meisel, Andreas UNSPECIFIED orcid.org/0000-0001-7233-5342 UNSPECIFIED Mergenthaler, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-217316
DOI:	10.3389/fnmol.2017.00305
Journal or Publication Title:	Front. Molec. Neurosci.
Volume:	10
Date:	2017
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1662-5099
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BRAIN ENERGY-METABOLISM; SPREADING DEPOLARIZATIONS; HIPPOCAMPAL-NEURONS; AEROBIC GLYCOLYSIS; ISCHEMIC-STROKE; IN-VITRO; OXYGEN; ASTROCYTES; GLUCOSE; CELLS Multiple languages Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21731