Universität zu Köln

Seppey, Christophe V. W., Singer, David ORCID: 0000-0002-4116-033X, Dumack, Kenneth ORCID: 0000-0001-8798-0483, Fournier, Bertrand ORCID: 0000-0002-7106-6109, Belbahri, Lassadd, Mitchell, Edward A. D. and Lara, Enrique ORCID: 0000-0001-8500-522X (2017). Distribution patterns of soil microbial eukaryotes suggests widespread algivory by phagotrophic protists as an alternative pathway for nutrient cycling. Soil Biol. Biochem., 112. S. 68 - 77. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 0038-0717

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Abstract

High-throughput sequencing (HTS) of soil environmental DNA (eDNA) allows assessing the full diversity of soil micro-eukaryotes. The resulting operational taxonomic units (OTUs) can be assigned to potential taxonomic and functional identities using increasingly complete reference databases. HTS of soil eDNA is revealing a high diversity and abundance of potential eukaryovorous protists, thus challenging the paradigm of the predominantly bacterivorous function of soil phagotrophic protists (i.e. microbial loop). Using Illumina sequencing of soil eDNA and targeting the V9 region of the SSU rRNA gene, we investigated the taxonomic and functional diversities, distribution and co-occurrence patterns of soil micro-eukaryotes in three land-use categories: forests, meadows and croplands located in Switzerland. Each CHU was assigned to a broad functional category (phototrophs, phagotrophs, osmotrophs, or parasites). Total OTU richness was similar in the three land-use categories, but community composition differed significantly between forests and other land-uses. The proportion of fungal sequences (especially Basidiomycota) was highest, and phototroph (i.e. soil microalgae) sequences least abundant in forests. Seven OTUs representing phagotrophic protists, together accounting for >25% of all phagotroph sequences, were significantly correlated to the total number of phototroph sequences, thus suggesting algivory. At least three of these OTUs corresponded to known algal predators. These results suggest that beyond plants, soil microalgae represent a functionally significant but rarely considered input of carbon in soils that should be taken into account when modelling soil nutrient cycling. (C) 2017 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Seppey, Christophe V. W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Singer, David UNSPECIFIED orcid.org/0000-0002-4116-033X UNSPECIFIED Dumack, Kenneth UNSPECIFIED orcid.org/0000-0001-8798-0483 UNSPECIFIED Fournier, Bertrand UNSPECIFIED orcid.org/0000-0002-7106-6109 UNSPECIFIED Belbahri, Lassadd UNSPECIFIED UNSPECIFIED UNSPECIFIED Mitchell, Edward A. D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lara, Enrique UNSPECIFIED orcid.org/0000-0001-8500-522X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-221296
DOI:	10.1016/j.soilbio.2017.05.002
Journal or Publication Title:	Soil Biol. Biochem.
Volume:	112
Page Range:	S. 68 - 77
Date:	2017
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	0038-0717
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FOOD SELECTION; HIGH DIVERSITY; COMMUNITY; CERCOZOA; PROTOZOA; RHIZARIA; CULTURE; NOV; CHLAMYDOPHRYIDAE; GLISSOMONADIDA Multiple languages Soil Science Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22129