Universität zu Köln

Pees, Barbara, Kloock, Anke ORCID: 0000-0002-4618-148X, Nakad, Rania, Barbosa, Camilo ORCID: 0000-0003-0433-9311 and Dierking, Katja ORCID: 0000-0002-5129-346X (2017). Enhanced behavioral immune defenses in a C. elegans C-type lectin-like domain gene mutant. Dev. Comp. Immunol., 74. S. 237 - 243. OXFORD: ELSEVIER SCI LTD. ISSN 1879-0089

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Abstract

C-type lectin-like domain (CTLD) proteins occupy crucial functions in the immune system of vertebrates, but their role in invertebrate immunity is much less understood. The nematode Caenorhabditis elegans possesses a highly diverse CTLD protein encoding (clec) gene repertoire. A role of C. elegans clec genes in pathogen defense is always assumed, yet experimental evidence for clec immune function is rare. To systematically test the potential function of clec genes in the C elegans defense against pathogens, we screened 39 clec mutants for survival on the Gram-positive pathogen Bacillus thuringiensis (BT18247) and 37 clec mutants on the Gram-negative pathogen Pseudomonas aeruginosa (PA14). We found that clec mutants can exhibit either decreased or, unexpectedly, increased resistance to infection. Since we observed high escape behavior for some of the clec mutants on BT18247 during the initial screen, we then asked if increased pathogen avoidance behavior underlies the increased resistance of some clec mutants. We thus tested lawn leaving behavior of the resistant clec-29(ok3181), clec-34(ok2120), clec151(ok2264), and C54G4.4(ok2110) mutant on BT18247. We found that C54G4.4(ok2110) mutant animals exhibit a particularly strong lawn leaving behavior, in addition to prolonged feeding cessation when exposed to BT18247. Together, our results indicate that clec genes mediate both resistance and susceptibility to infection. Further, behavioral analyses of the C54G4.4(ok2110) mutant implicate C54G4.4 in the regulation of pathogen avoidance behavior towards BT18247. We conclude that C elegans clec genes may act both as positive and negative regulators of physiological as well as behavioral immune defense responses. (C) 2017 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pees, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Kloock, Anke UNSPECIFIED orcid.org/0000-0002-4618-148X UNSPECIFIED Nakad, Rania UNSPECIFIED UNSPECIFIED UNSPECIFIED Barbosa, Camilo UNSPECIFIED orcid.org/0000-0003-0433-9311 UNSPECIFIED Dierking, Katja UNSPECIFIED orcid.org/0000-0002-5129-346X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-221328
DOI:	10.1016/j.dci.2017.04.021
Journal or Publication Title:	Dev. Comp. Immunol.
Volume:	74
Page Range:	S. 237 - 243
Date:	2017
Publisher:	ELSEVIER SCI LTD
Place of Publication:	OXFORD
ISSN:	1879-0089
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CAENORHABDITIS-ELEGANS; SEA-URCHIN; MULTIPLE; RECOGNITION; PATHOGEN; REPRODUCTION; ESTABLISHES; INFECTION; PROTEINS; SURVIVAL Multiple languages Fisheries; Immunology; Zoology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22132