Universität zu Köln

Zhao, Zhihan, Chen, Shixing, Wang, Jianbang, Su, Jing, Xu, Jiaqiang, Mathur, Sanjay, Fan, Chunhai ORCID: 0000-0002-7171-7338 and Song, Shiping (2017). Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing. Biosens. Bioelectron., 94. S. 605 - 609. OXFORD: ELSEVIER ADVANCED TECHNOLOGY. ISSN 1873-4235

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Abstract

Ultrasensitive biosensing technologies without gene amplification held great promise for direct detection of DNA. Herein we report a novel biosensing method, combining target recycling signal-amplification strategy and a homemade electrochemical device. Especially, the target recycling was achieved by a strand displacement process, no needing the help of any nucleases. In the presence of target DNA, the recycling system could be activated to generate a cascade of assembly steps with three hairpin DNA segments. Each recycling process were accompanied by a disassembly step that the last hairpin DNA segment displaces target DNA from the complex at the end of each circulation, freeing targets to activate the self-assembly of more trefoil DNA structures. This biosensing method could detect target DNA at aM level and can distinguish target DNA from interfering DNAs, demonstrating its high sensitivity and high selectivity. Importantly, the biosensing method could work well with serum samples.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zhao, Zhihan UNSPECIFIED UNSPECIFIED UNSPECIFIED Chen, Shixing UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Jianbang UNSPECIFIED UNSPECIFIED UNSPECIFIED Su, Jing UNSPECIFIED UNSPECIFIED UNSPECIFIED Xu, Jiaqiang UNSPECIFIED UNSPECIFIED UNSPECIFIED Mathur, Sanjay UNSPECIFIED UNSPECIFIED UNSPECIFIED Fan, Chunhai UNSPECIFIED orcid.org/0000-0002-7171-7338 UNSPECIFIED Song, Shiping UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-221759
DOI:	10.1016/j.bios.2017.03.051
Journal or Publication Title:	Biosens. Bioelectron.
Volume:	94
Page Range:	S. 605 - 609
Date:	2017
Publisher:	ELSEVIER ADVANCED TECHNOLOGY
Place of Publication:	OXFORD
ISSN:	1873-4235
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Inorganic Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LABEL-FREE; CHEMILUMINESCENCE DETECTION; STRATEGY; ACID Multiple languages Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22175