Universität zu Köln

Cauble, Meagan ORCID: 0000-0002-1481-8642, Yang, Phillip, Baumann, Ulrich, Gebauer, Jan M., Orr, Bradford G., Duong, Le T. and Holl, Mark M. Banaszak (2017). Microstructure dependent binding of pigment epithelium derived factor (PEDF) to type I collagen fibrils. J. Struct. Biol., 199 (2). S. 132 - 140. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1095-8657

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Abstract

Pigment epithelium derived factor (PEDF) is a multifunctional extracellular protein. In addition to its known anti-angiogenic and neurotrophic roles in collagen rich tissues, PEDF is thought to be involved in collagen fibril assembly due to its sequence specific binding to the collagen fibril and high expression in regions of active bone formation. In order to image the presence of the protein on the fibrils, PEDF was recombinantly made with a strep tag (strep-PEDF) and then gold nanoparticles conjugated to streptavidin (Au-NP) were used as a secondary tag. The gold nanoparticles were detected using phase imaging in tapping mode AFM to image where exogenous PEDF bound in rabbit femur. These findings demonstrate that PEDF binds heterogeneously in cortical rabbit femur. Exogenous PEDF binding was concentrated at areas between microstructures with highly aligned collagen fibrils. Binding was not observed on or within the collagen fibrils themselves. (C) 2017 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Cauble, Meagan UNSPECIFIED orcid.org/0000-0002-1481-8642 UNSPECIFIED Yang, Phillip UNSPECIFIED UNSPECIFIED UNSPECIFIED Baumann, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Gebauer, Jan M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Orr, Bradford G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Duong, Le T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Holl, Mark M. Banaszak UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-223230
DOI:	10.1016/j.jsb.2017.06.001
Journal or Publication Title:	J. Struct. Biol.
Volume:	199
Number:	2
Page Range:	S. 132 - 140
Date:	2017
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1095-8657
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IMPERFECTA TYPE VI; OSTEOGENESIS IMPERFECTA; BONE; MINERALIZATION; EXPRESSION; MUTATIONS; SERPINF1 Multiple languages Biochemistry & Molecular Biology; Biophysics; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22323