Universität zu Köln

Jeuck, Alexandra, Nitsche, Frank, Wylezich, Claudia ORCID: 0000-0002-0436-4480, Wirth, Olaf, Bergfeld, Tanja, Brutscher, Fabienne, Hennemann, Melanie, Monir, Shahla, Scherwass, Anja, Troll, Nicole and Arndt, Hartmut ORCID: 0000-0003-2811-3595 (2017). A Comparison of Methods to Analyze Aquatic Heterotrophic Flagellates of Different Taxonomic Groups. Protist, 168 (4). S. 375 - 392. MUNICH: ELSEVIER GMBH. ISSN 1434-4610

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Abstract

Heterotrophic flagellates contribute significantly to the matter flux in aquatic and terrestrial ecosystems. Still today their quantification and taxonomic classification bear several problems in field studies, though these methodological problems seem to be increasingly ignored in current ecological studies. Here we describe and test different methods, the live-counting technique, different fixation techniques, cultivation methods like the liquid aliquot method (LAM), and a molecular single cell survey called aliquot PCR (aPCR). All these methods have been tested either using aquatic field samples or cultures of freshwater and marine taxa. Each of the described methods has its advantages and disadvantages, which have to be considered in every single case. With the live-counting technique a detection of living cells up to morphospecies level is possible. Fixation of cells and staining methods are advantageous due to the possible long-term storage and observation of samples. Cultivation methods (LAM) offer the possibility of subsequent molecular analyses, and aPCR tools might complete the deficiency of LAM in terms of the missing detection of non-cultivable flagellates. In summary, we propose a combination of several investigation techniques reducing the gap between the different methodological problems. (C) 2017 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jeuck, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Nitsche, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Wylezich, Claudia UNSPECIFIED orcid.org/0000-0002-0436-4480 UNSPECIFIED Wirth, Olaf UNSPECIFIED UNSPECIFIED UNSPECIFIED Bergfeld, Tanja UNSPECIFIED UNSPECIFIED UNSPECIFIED Brutscher, Fabienne UNSPECIFIED UNSPECIFIED UNSPECIFIED Hennemann, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Monir, Shahla UNSPECIFIED UNSPECIFIED UNSPECIFIED Scherwass, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Troll, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Arndt, Hartmut UNSPECIFIED orcid.org/0000-0003-2811-3595 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-224308
DOI:	10.1016/j.protis.2017.04.003
Journal or Publication Title:	Protist
Volume:	168
Number:	4
Page Range:	S. 375 - 392
Date:	2017
Publisher:	ELSEVIER GMBH
Place of Publication:	MUNICH
ISSN:	1434-4610
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-VOLUME; EPIFLUORESCENCE MICROSCOPY; MICROBIAL EUKARYOTES; PROTIST DIVERSITY; CILIATES PROTOZOA; WATER-COLUMN; SEA; PCR; COMMUNITY; ABUNDANCE Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22430