Universität zu Köln

Ragionieri, Lapo, Ozbagci, Burak, Neupert, Susanne, Salts, Yuval, Davidovitch, Michael, Altstein, Miriam and Predel, Reinhard (2017). Identification of mature peptides from pban and capa genes of the moths Heliothis peltigera and Spodoptera littoralis. Peptides, 94. S. 1 - 10. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1873-5169

Full text not available from this repository.

Abstract

By transcriptome analysis, we identified PBAN and CAPA precursors in the moths Spodoptera littoralis and Heliothis peltigera which are among the most damaging pests of agriculture in tropical and subtropical Africa as well as in Mediterranean countries. A combination of mass spectrometry and immunocytochemistry was used to identify mature peptides processed from these precursors and to reveal their spatial distribution in the CNS. We found that the sites of expression of pban genes, the structure of PBAN precursors and the processed neuro-peptides are very similar in noctuid moths. The sequence of the diapause hormone (DH; tryptopyrokinin following the signal peptide), however, contains two N-terminal amino acids more than expected from comparison with already published sequences of related species. Capa genes of S. littoralis and H. peltigera encode, in addition to periviscerokinins, a tryptopyrokinin showing sequence similarity with DH, which is the tryptopyrokinin of the pban gene. CAPA peptides, which were not known from any noctuid moth so far, are produced in cells of abdominal ganglia. The shape of the release sites of these hormones in H. peltigera represents an exceptionally derived trait state and does not resemble the well-structured abdominal perisympathetic organs which are known from many other insects. Instead, axons of CAPA cells extensively ramify within the ventral diaphragm. The novel information regarding the sequences of all mature peptides derived from pban and capa genes of H. peltigera and S. littoralis now enables a detailed analysis of the bioactivity and species-specificity of the native peptides, especially those from the hitherto unknown capa genes, and to explore their interactions with PBAN/DH receptors.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ragionieri, Lapo UNSPECIFIED UNSPECIFIED UNSPECIFIED Ozbagci, Burak UNSPECIFIED UNSPECIFIED UNSPECIFIED Neupert, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Salts, Yuval UNSPECIFIED UNSPECIFIED UNSPECIFIED Davidovitch, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Altstein, Miriam UNSPECIFIED UNSPECIFIED UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-224395
DOI:	10.1016/j.peptides.2017.05.004
Journal or Publication Title:	Peptides
Volume:	94
Page Range:	S. 1 - 10
Date:	2017
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1873-5169
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BIOSYNTHESIS-ACTIVATING NEUROPEPTIDE; PHEROMONE BIOSYNTHESIS; PERISYMPATHETIC ORGANS; TOBACCO HAWKMOTH; MANDUCA-SEXTA; MYOTROPIC NEUROPEPTIDE; SUBESOPHAGEAL GANGLION; FUNCTIONAL EXPRESSION; MOLECULAR-CLONING; DIAPAUSE HORMONE Multiple languages Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22439