Universität zu Köln

Wu, Beilei, Zwart, Mark P., Sanchez-Navarro, Jesus A. and Elena, Santiago F. ORCID: 0000-0001-8249-5593 (2017). Within-host Evolution of Segments Ratio for the Tripartite Genome of Alfalfa Mosaic Virus. Sci Rep, 7. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

The existence of multipartite viruses is an intriguing mystery in evolutionary virology. Several hypotheses suggest benefits that should outweigh the costs of a reduced transmission efficiency and of segregation of coadapted genes associated with encapsidating each segment into a different particle. Advantages range from increasing genome size despite high mutation rates, faster replication, more efficient selection resulting from reassortment during mixed infections, better regulation of gene expression, or enhanced virion stability and cell-to-cell movement. However, support for these hypotheses is scarce. Here we report experiments testing whether an evolutionary stable equilibrium exists for the three genomic RNAs of Alfalfa mosaic virus (AMV). Starting infections with different segment combinations, we found that the relative abundance of each segment evolves towards a constant ratio. Population genetic analyses show that the segment ratio at this equilibrium is determined by frequency-dependent selection. Replication of RNAs 1 and 2 was coupled and collaborative, whereas the replication of RNA 3 interfered with the replication of the other two. We found that the equilibrium solution is slightly different for the total amounts of RNA produced and encapsidated, suggesting that competition exists between all RNAs during encapsidation. Finally, we found that the observed equilibrium appears to be host-species dependent.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wu, Beilei UNSPECIFIED UNSPECIFIED UNSPECIFIED Zwart, Mark P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sanchez-Navarro, Jesus A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Elena, Santiago F. UNSPECIFIED orcid.org/0000-0001-8249-5593 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-225059
DOI:	10.1038/s41598-017-05335-8
Journal or Publication Title:	Sci Rep
Volume:	7
Date:	2017
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DEPENDENT RNA-POLYMERASE; TRANS-ACTING FUNCTIONS; COAT PROTEIN; VIRAL-RNA; COPY NUMBER; REPLICATION; CIS; BINDING; AMPLIFICATION; TRANSLATION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22505