Universität zu Köln

Mehling, Lena-Maria, Piper, Thomas ORCID: 0000-0002-7462-6693, Spottke, Annika, Heidbreder, Anna, Young, Peter, Madea, Burkhard, Thevis, Mario and Hess, Cornelius (2017). GHB-O-beta-glucuronide in blood and urine is not a suitable tool for the extension of the detection window after GHB intake. Forensic Toxicol., 35 (2). S. 263 - 275. NEW YORK: SPRINGER. ISSN 1860-8973

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Abstract

Because of its small detection window, uncovering drug-facilitated crime after gamma-hydroxybutyric acid (GHB) intake remains a problem. The aim of this experiment was to determine endogenous concentrations of GHB and GHB-O-beta-glucuronide (GHB-Gluc) in plasma and urine samples and to compare them with concentrations after GHB intake in humans. Plasma and urine samples of volunteers (n = 50) who had never taken GHB during their lifetime (control group) were collected, and endogenous concentrations of GHB and GHB-Gluc were determined. In addition, plasma and urine samples of patients (n = 3) therapeutically taking sodium oxybate (GHB-sodium salt) were collected prior to and at different time points after the intake. GHB was determined via a liquid chromatography (LC)-tandem mass spectrometry system operated in multiple reaction monitoring mode. GHB-Gluc was detected by LC-quadrupole time-of-flight mass spectrometry. In plasma and urine samples of the control group (n = 50), endogenous concentrations of GHB-Gluc ranged from 0.011 to 0.067 mg/L and from 0.16 to 7.1 mg/L, respectively, while unconjugated GHB concentrations were less than 2 mg/L in both matrices. In contrast, after sodium oxybate administration, GHB concentrations increased markedly, and fell to below the commonly used cutoff value (plasma 4 mg/L and urine 10 mg/L) after 6-8 h in all patients. GHB-Gluc concentrations showed no significant time-dependent increase in plasma samples. In urine, GHB-Gluc concentrations increased after GHB intake, but were generally not higher than the endogenous concentrations of the control group. Therefore, it can be concluded that GHB-Gluc concentrations are not a suitable marker for extending the detection window after GHB intake.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mehling, Lena-Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Piper, Thomas UNSPECIFIED orcid.org/0000-0002-7462-6693 UNSPECIFIED Spottke, Annika UNSPECIFIED UNSPECIFIED UNSPECIFIED Heidbreder, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Young, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Madea, Burkhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Hess, Cornelius UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-226362
DOI:	10.1007/s11419-016-0352-7
Journal or Publication Title:	Forensic Toxicol.
Volume:	35
Number:	2
Page Range:	S. 263 - 275
Date:	2017
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1860-8973
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GAMMA-HYDROXYBUTYRIC ACID; PHARMACOKINETICS; NARCOLEPSY; HUMANS; SLEEP; METABOLITE; CATAPLEXY; ECSTASY; DRUG Multiple languages Toxicology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22636