Universität zu Köln

Casas-Vila, Nuria, Bluhm, Alina, Sayols, Sergi ORCID: 0000-0002-3877-4170, Dinges, Nadja, Dejung, Mario, Altenhein, Tina, Kappei, Dennis ORCID: 0000-0002-3582-2253, Altenhein, Benjamin, Roignant, Jean-Yves ORCID: 0000-0003-1395-2150 and Butter, Falk ORCID: 0000-0002-7197-7279 (2017). The developmental proteome of Drosophila melanogaster. Genome Res., 27 (7). S. 1273 - 1286. COLD SPRING HARBOR: COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT. ISSN 1549-5469

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Abstract

Drosophila melanogaster is a widely used genetic model organism in developmental biology. While this model organism has been intensively studied at the RNA level, a comprehensive proteomic study covering the complete life cycle is still missing. Here, we apply label-free quantitative proteomics to explore proteome remodeling across Drosophila's life cycle, resulting in 7952 proteins, and provide a high temporal-resolved embryogenesis proteome of 5458 proteins. Our proteome data enabled us to monitor isoform-specific expression of 34 genes during development, to identify the pseudogene Cyp9f3. as a protein-coding gene, and to obtain evidence of 268 small proteins. Moreover, the comparison with available transcriptomic data uncovered examples of poor correlation between mRNA and protein, underscoring the importance of proteomics to study developmental progression. Data integration of our embryogenesis proteome with tissue-specific data revealed spatial and temporal information for further functional studies of yet uncharacterized proteins. Overall, our high resolution proteomes provide a powerful resource and can be explored in detail in our interactive web interface.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Casas-Vila, Nuria UNSPECIFIED UNSPECIFIED UNSPECIFIED Bluhm, Alina UNSPECIFIED UNSPECIFIED UNSPECIFIED Sayols, Sergi UNSPECIFIED orcid.org/0000-0002-3877-4170 UNSPECIFIED Dinges, Nadja UNSPECIFIED UNSPECIFIED UNSPECIFIED Dejung, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Altenhein, Tina UNSPECIFIED UNSPECIFIED UNSPECIFIED Kappei, Dennis UNSPECIFIED orcid.org/0000-0002-3582-2253 UNSPECIFIED Altenhein, Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Roignant, Jean-Yves UNSPECIFIED orcid.org/0000-0003-1395-2150 UNSPECIFIED Butter, Falk UNSPECIFIED orcid.org/0000-0002-7197-7279 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-226612
DOI:	10.1101/gr.213694.116
Journal or Publication Title:	Genome Res.
Volume:	27
Number:	7
Page Range:	S. 1273 - 1286
Date:	2017
Publisher:	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
Place of Publication:	COLD SPRING HARBOR
ISSN:	1549-5469
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OPEN READING FRAMES; MESSENGER-RNA; GENE-EXPRESSION; QUANTITATIVE PROTEOMICS; EMBRYONIC-DEVELOPMENT; OOCYTE MATURATION; GLOBAL ANALYSIS; PROTEINS; QUANTIFICATION; IDENTIFICATION Multiple languages Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22661