Universität zu Köln

Schiffner, Julia, Roos, Judith, Broomhead, David, van Helden, Joseph, Godehardt, Erhard, Fehr, Daniel, Freundl, Guenter, Johnson, Sarah and Gnoth, Christian (2017). Relationship between anti-Mullerian hormone and antral follicle count across the menstrual cycle using the Beckman Coulter Access assay in comparison with Gen II manual assay. Clin. Chem. Lab. Med., 55 (7). S. 1025 - 1034. BERLIN: WALTER DE GRUYTER GMBH. ISSN 1437-4331

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Abstract

Background: The study aim was to validate Beckman Coulter's fully automated Access Immunoassay System (BC Access assay) for anti-Mullerian hormone (AMH) and compare it with Beckman Coulter's Modified Manual Generation II assay (BC Mod Gen II), with regard to cycle AMH fluctuations and antral follicle counts. Methods: During one complete menstrual cycle, transvaginal ultrasound was performed on regularly menstruating women (n = 39; 18-40 years) every 2 days until the dominant ovarian follicle reached 16 mm, then daily until observed ovulation; blood samples were collected throughout the cycle. Number and size of antral follicles was determined and AMH levels measured using both assays. Results: AMH levels measured by the BC Access assay vary over ovulatory menstrual cycles, with a statistically significant pre-ovulatory decrease from -5 to +2 days around objective ovulation. Mean luteal AMH levels were significantly lower (- 7.99%) than mean follicular levels but increased again towards the end of the luteal phase. Antral follicle count can be estimated from AMH (ng/mL, BC Access assay) concentrations on any follicular phase day. BC Access assay-obtained AMH values are considerably lower compared with the BC Mod Gen II assay (- 19% on average); conversion equation: AMH BC Access (ng/mL) = 0.85 [AMH BC Mod Gen II (ng/mL)](0.95). Conclusions: AMH levels vary throughout the cycle, independently of assay utilised. A formula can be used to convert BC Access assay-obtained AMH levels to BC Mod Gen II values. The number of antral follicles can be consistently estimated from pre-ovulatory AMH levels using either assay.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schiffner, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Roos, Judith UNSPECIFIED UNSPECIFIED UNSPECIFIED Broomhead, David UNSPECIFIED UNSPECIFIED UNSPECIFIED van Helden, Joseph UNSPECIFIED UNSPECIFIED UNSPECIFIED Godehardt, Erhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Fehr, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Freundl, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED Johnson, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Gnoth, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-226981
DOI:	10.1515/cclm-2016-0609
Journal or Publication Title:	Clin. Chem. Lab. Med.
Volume:	55
Number:	7
Page Range:	S. 1025 - 1034
Date:	2017
Publisher:	WALTER DE GRUYTER GMBH
Place of Publication:	BERLIN
ISSN:	1437-4331
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENZYME-LINKED-IMMUNOSORBENT; MLLERIAN HORMONE; URINARY Multiple languages Medical Laboratory Technology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22698