Universität zu Köln

Wawra, Stephan ORCID: 0000-0001-8555-1618, Trusch, Franziska ORCID: 0000-0003-0608-202X, Matena, Anja, Apostolakis, Kostis, Linne, Uwe, Zhukov, Igor, Stanek, Jan ORCID: 0000-0003-1660-168X, Kozminski, Wiktor ORCID: 0000-0003-2319-4525, Davidson, Ian, Secombes, Chris J., Bayer, Peter ORCID: 0000-0003-0435-7202 and van Westa, Pieter (2017). The RxLR Motif of the Host Targeting Effector AVR3a of Phytophthora infestans Is Cleaved before Secretion. Plant Cell, 29 (6). S. 1184 - 1196. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1531-298X

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Abstract

When plant-pathogenic oomycetes infect their hosts, they employ a large arsenal of effector proteins to establish a successful infection. Some effector proteins are secreted and are destined to be translocated and function inside host cells. The largest group of translocated proteins from oomycetes is the RxLR effectors, defined by their conserved N-terminal Arg-Xaa-Leu-Arg (RxLR) motif. However, the precise role of this motif in the host cell translocation process is unclear. Here, detailed biochemical studies of the RxLR effector AVR3a from the potato pathogen Phytophthora infestans are presented. Mass spectrometric analysis revealed that the RxLR sequence of native AVR3a is cleaved off prior to secretion by the pathogen and the N terminus of the mature effector was found likely to be acetylated. High-resolution NMR structure analysis of AVR3a indicates that the RxLR motif is well accessible to potential processing enzymes. Processing and modification of AVR3a is to some extent similar to events occurring with the export element (PEXEL) found in malaria effector proteins from Plasmodium falciparum. These findings imply a role for the RxLR motif in the secretion of AVR3a by the pathogen, rather than a direct role in the host cell entry process itself.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wawra, Stephan UNSPECIFIED orcid.org/0000-0001-8555-1618 UNSPECIFIED Trusch, Franziska UNSPECIFIED orcid.org/0000-0003-0608-202X UNSPECIFIED Matena, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Apostolakis, Kostis UNSPECIFIED UNSPECIFIED UNSPECIFIED Linne, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhukov, Igor UNSPECIFIED UNSPECIFIED UNSPECIFIED Stanek, Jan UNSPECIFIED orcid.org/0000-0003-1660-168X UNSPECIFIED Kozminski, Wiktor UNSPECIFIED orcid.org/0000-0003-2319-4525 UNSPECIFIED Davidson, Ian UNSPECIFIED UNSPECIFIED UNSPECIFIED Secombes, Chris J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bayer, Peter UNSPECIFIED orcid.org/0000-0003-0435-7202 UNSPECIFIED van Westa, Pieter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-230186
DOI:	10.1105/tpc.16.00552
Journal or Publication Title:	Plant Cell
Volume:	29
Number:	6
Page Range:	S. 1184 - 1196
Date:	2017
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1531-298X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PHOSPHATIDYLINOSITOL 3-PHOSPHATE; HYALOPERONOSPORA-ARABIDOPSIDIS; MALARIA PROTEINS; STRUCTURAL BASIS; PLASMA-MEMBRANE; CELL-DEATH; PLANT; OOMYCETE; EXPORT; VIRULENCE Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23018