Universität zu Köln

Gao, Dong-Mei, Zhao, Yue, Chen, Jie, Zhao, Yan, Chen, Jun and Zhou, Hai-Jun (2017). Hepatocytes Express IL-2 In vitro in The Presence of Both HBx Stimulation and Mild Oxidative Stress. Prog. Biochem. Biophys., 44 (5). S. 422 - 431. BEIJING: CHINESE ACAD SCIENCES, INST BIOPHYSICS. ISSN 1000-3282

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Abstract

Hepatocellular carcinoma (HCC) has an extremely poor prognosis which is caused mainly by high frequency of metastatic recurrence. We previously reported that high interleukin (IL)-2 levels in peritumoral tissue were closely associated with a decreased incidence of intrahepatic tumor recurrence in patients with hepatitis B virus (HBV)-associated HCC, and the immune staining of IL-2 primarily was found in tumor-surrounding hepatocytes. However, whether hepatocytes can express IL-2 in vitro and underlying mechanism remain to be investigated. Here, we compared IL-2 expression levels in peritumoral tissue from HCC patients between HBV-positive group and HBV-negative group. We then overexpressed HBV x protein (HBx) in immortalized human liver cell line THLE-2, and mimic microenvironmental oxidative stress with hydrogen peroxide (H2O2) treatment in vitro. We demonstrated that hepatocytes can express IL-2 in vitro through MAP3K7/NF-kappa B pathway in the presence of both HBx stimulation and mild oxidative stress. Targeting microenvironmental oxidative stress offers a promise for prevention and therapy of HBV-associated HCC metastatic recurrence.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gao, Dong-Mei UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhao, Yue UNSPECIFIED UNSPECIFIED UNSPECIFIED Chen, Jie UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhao, Yan UNSPECIFIED UNSPECIFIED UNSPECIFIED Chen, Jun UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhou, Hai-Jun UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-232259
DOI:	10.16476/j.pibb.2017.0036
Journal or Publication Title:	Prog. Biochem. Biophys.
Volume:	44
Number:	5
Page Range:	S. 422 - 431
Date:	2017
Publisher:	CHINESE ACAD SCIENCES, INST BIOPHYSICS
Place of Publication:	BEIJING
ISSN:	1000-3282
Language:	English
Faculty:	Faculty of Medicine
Divisions:	Faculty of Medicine > Chirurgie > Klinik und Poliklinik für Allgemein-, Viszeral- und Tumorchirurgie
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHRONIC HEPATITIS-C; HUMAN HEPATOCELLULAR-CARCINOMA; CANCER; INTERLEUKIN-2; METASTASIS; GROWTH; CELLS; PROLIFERATION; ACTIVATION; INFECTION Multiple languages Biochemistry & Molecular Biology; Biophysics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23225