Universität zu Köln

Breddermann, Hannes and Schnetz, Karin ORCID: 0000-0003-4693-6202 (2017). Activation of leuO by LrhA in Escherichia coli. Mol. Microbiol., 104 (4). S. 664 - 677. HOBOKEN: WILEY. ISSN 1365-2958

Full text not available from this repository.

Abstract

LeuO is a conserved LysR-type transcription factor of pleiotropic function in Enterobacteria. Regulation of the leuO gene has been best studied in Escherichia coli and Salmonella enterica. Its expression is silenced by the nucleoid-associated proteins H-NS and StpA, autoregulated by LeuO, and in E. coli activated by the transcription regulator BglJ-RcsB. However, signals which induce leuO expression remain unknown. Here we show that LrhA, a conserved LysR-type transcription regulator, activates leuO in E. coli. LrhA specifically binds the leuO regulatory region and activates expression of leuO from three promoters. Activation of leuO by LrhA is synergistic with activation by BglJ-RcsB, while co-regulation by LrhA, LeuO and H-NS/StpA suggests a complex regulatory interplay. In addition, hyperactive LrhA mutants including LrhA-12DN, 221TA, 61HR/221TA and 303DG were identified. Regulation of leuO by LrhA reveals a connection between the two pleiotropic regulators LeuO and LrhA in E. coli.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Breddermann, Hannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Schnetz, Karin UNSPECIFIED orcid.org/0000-0003-4693-6202 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-232452
DOI:	10.1111/mmi.13656
Journal or Publication Title:	Mol. Microbiol.
Volume:	104
Number:	4
Page Range:	S. 664 - 677
Date:	2017
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-2958
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LYSR-TYPE REGULATOR; ENTERICA SEROVAR TYPHI; H-NS; TRANSCRIPTIONAL REGULATION; GLOBAL REGULATOR; BGL OPERON; YERSINIA-PSEUDOTUBERCULOSIS; VIRULENCE DETERMINANTS; RCS PHOSPHORELAY; BINDING DOMAIN Multiple languages Biochemistry & Molecular Biology; Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23245