Universität zu Köln

Ribbe, Jan, Baker, Amy E., Euler, Sebastian ORCID: 0000-0001-9583-9009, O'Toole, George A. and Maier, Berenike ORCID: 0000-0001-6971-9927 (2017). Role of Cyclic Di-GMP and Exopolysaccharide in Type IV Pilus Dynamics. J. Bacteriol., 199 (8). WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-5530

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Abstract

For Pseudomonas aeruginosa, levels of cyclic di-GMP (c-di-GMP) govern the transition from the planktonic state to biofilm formation. Type IV pili (T4P) are crucial determinants of biofilm structure and dynamics, but it is unknown how levels of c-di-GMP affect pilus dynamics. Here, we scrutinized how c-di-GMP affects molecular motor properties and adhesive behavior of T4P. By means of retraction, T4P generated forces of similar to 30 pN. Deletion mutants in the proteins with known roles in biofilm formation, swarming motility, and exopolysaccharide (EPS) production (specifically, the diguanylate cyclases sadC and roeA or the c-di-GMP phosphodiesterase bifA) showed only modest effects on velocity or force of T4P retraction. At high levels of c-di-GMP, the production of exopolysaccharides, particularly of Pel, is upregu-lated. We found that Pel production strongly enhances T4P-mediated surface adhesion of P. aeruginosa, suggesting that T4P-matrix interactions may be involved in biofilm formation by P. aeruginosa. Finally, our data support the previously proposed model of slingshot-like twitching motility of P. aeruginosa. IMPORTANCE Type IV pili (T4P) play various important roles in the transition of bacteria from the planktonic state to the biofilm state, including surface attachment and surface sensing. Here, we investigate adhesion, dynamics, and force generation of T4P after bacteria engage a surface. Our studies showed that two critical components of biofilm formation by Pseudomonas aeruginosa, T4P and exopolysaccharides, contribute to enhanced T4P-mediated force generation by attached bacteria. These data indicate a crucial role for the coordinated impact of multiple biofilm-promoting factors during the early stages of attachment to a surface. Our data are also consistent with a previous model explaining why pilus-mediated motility in P. aeruginosa results in characteristic twitching behavior.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ribbe, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Baker, Amy E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Euler, Sebastian UNSPECIFIED orcid.org/0000-0001-9583-9009 UNSPECIFIED O'Toole, George A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maier, Berenike UNSPECIFIED orcid.org/0000-0001-6971-9927 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-235117
DOI:	10.1128/JB.00859-16
Journal or Publication Title:	J. Bacteriol.
Volume:	199
Number:	8
Date:	2017
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-5530
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Physics > Institut für Biologische Physik
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PSEUDOMONAS-AERUGINOSA; NEISSERIA-GONORRHOEAE; NONOPSONIC PHAGOCYTOSIS; SWARMING MOTILITY; BIOFILM FORMATION; RETRACTION; ATTACHMENT; GENES; IDENTIFICATION; SURFACES Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23511