Universität zu Köln

Misas-Villamil, Johana C., van der Burgh, Aranka M., Grosse-Holz, Friederike, Bach-Pages, Marcel ORCID: 0000-0002-9685-737X, Kovacs, Judit, Kaschani, Farnusch, Schilasky, Soeren, Emon, Asif E. K., Ruben, Mark, Kaiser, Markus, Overkleeft, Hermen S. and van der Hoorn, Renier A. L. (2017). Subunit-selective proteasome activity profiling uncovers uncoupled proteasome subunit activities during bacterial infections. Plant J., 90 (2). S. 418 - 431. HOBOKEN: WILEY. ISSN 1365-313X

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Abstract

The proteasome is a nuclear-cytoplasmic proteolytic complex involved in nearly all regulatory pathways in plant cells. The three different catalytic activities of the proteasome can have different functions, but tools to monitor and control these subunits selectively are not yet available in plant science. Here, we introduce subunit-selective inhibitors and dual-color fluorescent activity-based probes for studying two of the three active catalytic subunits of the plant proteasome. We validate these tools in two model plants and use this to study the proteasome during plant-microbe interactions. Our data reveal that Nicotiana benthamiana incorporates two different paralogs of each catalytic subunit into active proteasomes. Interestingly, both beta 1 and beta 5 activities are significantly increased upon infection with pathogenic Pseudomonas syringae pv. tomato DC3000 lacking hopQ1-1 [PtoDC3000(Delta hQ)] whilst the activity profile of the beta 1 subunit changes. Infection with wild-type PtoDC3000 causes proteasome activities that range from strongly induced beta 1 and beta 5 activities to strongly suppressed beta 5 activities, revealing that beta 1 and beta 5 activities can be uncoupled during bacterial infection. These selective probes and inhibitors are now available to the plant science community, and can be widely and easily applied to study the activity and role of the different catalytic subunits of the proteasome in different plant species.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Misas-Villamil, Johana C. UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Burgh, Aranka M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Grosse-Holz, Friederike UNSPECIFIED UNSPECIFIED UNSPECIFIED Bach-Pages, Marcel UNSPECIFIED orcid.org/0000-0002-9685-737X UNSPECIFIED Kovacs, Judit UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaschani, Farnusch UNSPECIFIED UNSPECIFIED UNSPECIFIED Schilasky, Soeren UNSPECIFIED UNSPECIFIED UNSPECIFIED Emon, Asif E. K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruben, Mark UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaiser, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Overkleeft, Hermen S. UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Hoorn, Renier A. L. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-235409
DOI:	10.1111/tpj.13494
Journal or Publication Title:	Plant J.
Volume:	90
Number:	2
Page Range:	S. 418 - 431
Date:	2017
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-313X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language 20S PROTEASOME; PSEUDOMONAS-SYRINGAE; CYSTEINE PROTEASES; PHYTOPHTHORA-INFESTANS; LEAF SENESCENCE; PROTEIN; DEFENSE; DISEASE; QUANTIFICATION; PURIFICATION Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23540