Universität zu Köln

Gabriel, Elke, Ramani, Anand, Karow, Ulrike, Gottardo, Marco ORCID: 0000-0001-8649-7799, Natarajan, Karthick, Gooi, Li Ming, Goranci-Buzhala, Gladiola, Krut, Oleg, Peters, Franziska, Nikolic, Milos ORCID: 0000-0003-0029-7601, Kuivanen, Suvi, Korhonen, Essi ORCID: 0000-0003-4730-7715, Smura, Teemu ORCID: 0000-0002-9187-3151, Vapalahti, Olli ORCID: 0000-0003-2270-6824, Papantonis, Argyris ORCID: 0000-0001-7551-1073, Schmidt-Chanasit, Jonas, Riparbelli, Maria, Callaini, Giuliano ORCID: 0000-0003-2252-0309, Kroenke, Martin, Utermoehlen, Olaf and Gopalakrishnan, Jay (2017). Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids. Cell Stem Cell, 20 (3). S. 397 - 412. CAMBRIDGE: CELL PRESS. ISSN 1875-9777

Full text not available from this repository.

Abstract

The recent Zika virus (ZIKV) epidemic is associated with microcephaly in newborns. Although the connection between ZIKV and neurodevelopmental defects is widely recognized, the underlying mechanisms are poorly understood. Here we show that two recently isolated strains of ZIKV, an American strain from an infected fetal brain (FB-GWUH-2016) and a closely-related Asian strain (H/PF/2013), productively infect human iPSC-derived brain organoids. Both of these strains readily target to and replicate in proliferating ventricular zone (VZ) apical progenitors. The main phenotypic effect was premature differentiation of neural progenitors associated with centrosome perturbation, even during early stages of infection, leading to progenitor depletion, disruption of the VZ, impaired neurogenesis, and cortical thinning. The infection pattern and cellular outcome differ from those seen with the extensively passaged ZIKV strain MR766. The structural changes we see after infection with these more recently isolated viral strains closely resemble those seen in ZIKV-associated microcephaly.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gabriel, Elke UNSPECIFIED UNSPECIFIED UNSPECIFIED Ramani, Anand UNSPECIFIED UNSPECIFIED UNSPECIFIED Karow, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED Gottardo, Marco UNSPECIFIED orcid.org/0000-0001-8649-7799 UNSPECIFIED Natarajan, Karthick UNSPECIFIED UNSPECIFIED UNSPECIFIED Gooi, Li Ming UNSPECIFIED UNSPECIFIED UNSPECIFIED Goranci-Buzhala, Gladiola UNSPECIFIED UNSPECIFIED UNSPECIFIED Krut, Oleg UNSPECIFIED UNSPECIFIED UNSPECIFIED Peters, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Nikolic, Milos UNSPECIFIED orcid.org/0000-0003-0029-7601 UNSPECIFIED Kuivanen, Suvi UNSPECIFIED UNSPECIFIED UNSPECIFIED Korhonen, Essi UNSPECIFIED orcid.org/0000-0003-4730-7715 UNSPECIFIED Smura, Teemu UNSPECIFIED orcid.org/0000-0002-9187-3151 UNSPECIFIED Vapalahti, Olli UNSPECIFIED orcid.org/0000-0003-2270-6824 UNSPECIFIED Papantonis, Argyris UNSPECIFIED orcid.org/0000-0001-7551-1073 UNSPECIFIED Schmidt-Chanasit, Jonas UNSPECIFIED UNSPECIFIED UNSPECIFIED Riparbelli, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Callaini, Giuliano UNSPECIFIED orcid.org/0000-0003-2252-0309 UNSPECIFIED Kroenke, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Utermoehlen, Olaf UNSPECIFIED UNSPECIFIED UNSPECIFIED Gopalakrishnan, Jay UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-236852
DOI:	10.1016/j.stem.2016.12.005
Journal or Publication Title:	Cell Stem Cell
Volume:	20
Number:	3
Page Range:	S. 397 - 412
Date:	2017
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1875-9777
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-CYCLE; CEREBRAL ORGANOIDS; MOUSE-BRAIN; STEM-CELLS; CEP152; MUTATIONS; INFECTION; NEURONS; BIOLOGY; MITOSIS Multiple languages Cell & Tissue Engineering; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23685