Universität zu Köln

Koroska, Florian, Goettig, Stephan, Kaase, Martin, Steinmann, Joerg, Gatermann, Soeren, Sommer, Julian, Wille, Thorsten, Plum, Georg and Hamprecht, Axel ORCID: 0000-0003-1449-5780 (2017). Comparison of Phenotypic Tests and an Immunochromatographic Assay and Development of a New Algorithm for Detection of OXA-48-like Carbapenemases. J. Clin. Microbiol., 55 (3). S. 877 - 884. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-660X

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Abstract

OXA-48 is the most prevalent carbapenemase in Enterobacteriaceae in Europe and the Middle East, but it is frequently missed because many isolates display low MICs for carbapenems. Furthermore, in contrast to metallo-beta-lactamases or Klebsiella pneumoniae carbapenemases (KPC), no specific inhibitor is available for the phenotypic detection of OXA-48. Molecular detection of blaOXA-48 is the gold standard but is not available in many laboratories. A few phenotypic assays have been described but have not been independently evaluated. The aim of this study was the systematic comparison of phenotypic tests and an immunochromatographic assay (ICT) for the detection of OXA-48/OXA-48-like carbapenemases and the development of an algorithm for reliable phenotypic detection of OXA-48. Four phenotypic tests (temocillin disk test, faropenem disk test, OXA-48 disk test, and high-inoculum [HI] OXA-48 disk test) and a new ICT (OXA-48 K-SeT) were compared by using a set of 166 Enterobacteriaceae isolates, including isolates producing OXA-48/OXA-48-like carbapenemases (n = 84) or Ambler class A and B carbapenemases (n = 41) and carbapenemase-negative isolates (n = 41). The sensitivity and specificity for the different assays were 100% and 43.9% for temocillin, 57.1% and 98.8% for faropenem, 53.6% and 100% for the OXA-48 disk test, 98.8% and 97.6% for the HI OXA-48 disk test, and 100% and 100% for the ICT, respectively. The ICT displayed the highest sensitivity and specificity and was the most rapid assay, but it is more costly than phenotypic assays. Based on these results, a new algorithm incorporating temocillin, faropenem, and ICT which allows cost-effective detection of OXA-48 with 100% sensitivity and specificity was developed.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koroska, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Goettig, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaase, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Steinmann, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Gatermann, Soeren UNSPECIFIED UNSPECIFIED UNSPECIFIED Sommer, Julian UNSPECIFIED UNSPECIFIED UNSPECIFIED Wille, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Plum, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamprecht, Axel UNSPECIFIED orcid.org/0000-0003-1449-5780 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-238053
DOI:	10.1128/JCM.01929-16
Journal or Publication Title:	J. Clin. Microbiol.
Volume:	55
Number:	3
Page Range:	S. 877 - 884
Date:	2017
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-660X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RAPID DETECTION; K-SET; ENTEROBACTERIACEAE; RESISTANCE; PREVALENCE; TEMOCILLIN; SPREAD; SPP.; KPC Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23805