Universität zu Köln

Flores-Tornero, Maria ORCID: 0000-0002-9296-0070, Anoman, Armand D., Rosa-Tellez, Sara, Toujani, Walid, Weber, Andreas P. M., Eisenhut, Marion, Kurz, Samantha, Alseekh, Saleh, Fernie, Alisdair R., Munoz-Bertomeu, Jesus and Ros, Roc ORCID: 0000-0003-4254-8368 (2017). Overexpression of the triose phosphate translocator (TPT) complements the abnormal metabolism and development of plastidial glycolytic glyceraldehyde-3-phosphate dehydrogenase mutants. Plant J., 89 (6). S. 1146 - 1159. HOBOKEN: WILEY. ISSN 1365-313X

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Abstract

The presence of two glycolytic pathways working in parallel in plastids and cytosol has complicated the understanding of this essential process in plant cells, especially the integration of the plastidial pathway into the metabolism of heterotrophic and autotrophic organs. It is assumed that this integration is achieved by transport systems, which exchange glycolytic intermediates across plastidial membranes. However, it is unknown whether plastidial and cytosolic pools of 3-phosphoglycerate (3-PGA) can equilibrate in non-photosynthetic tissues. To resolve this question, we employed Arabidopsis mutants of the plastidial glycolytic isoforms of glyceraldehyde-3-phosphate dehydrogenase (GAPCp) that express the triose phosphate translocator (TPT) under the control of the 35S (35S:TPT) or the native GAPCp1 (GAPCp1:TPT) promoters. TPT expression under the control of both promoters complemented the vegetative developmental defects and metabolic disorders of the GAPCp double mutants (gapcp1gapcp2). However, as the 35S is poorly expressed in the tapetum, full vegetative and reproductive complementation of gapcp1gapcp2 was achieved only by transforming this mutant with the GAPCp1: TPT construct. Our results indicate that the main function of GAPCp is to supply 3-PGA for anabolic pathways in plastids of heterotrophic cells and suggest that the plastidial glycolysis may contribute to fatty acid biosynthesis in seeds. They also suggest a 3-PGA deficiency in the plastids of gapcp1gapcp2, and that 3-PGA pools between cytosol and plastid do not equilibrate in heterotrophic cells.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Flores-Tornero, Maria UNSPECIFIED orcid.org/0000-0002-9296-0070 UNSPECIFIED Anoman, Armand D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rosa-Tellez, Sara UNSPECIFIED UNSPECIFIED UNSPECIFIED Toujani, Walid UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eisenhut, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Kurz, Samantha UNSPECIFIED UNSPECIFIED UNSPECIFIED Alseekh, Saleh UNSPECIFIED UNSPECIFIED UNSPECIFIED Fernie, Alisdair R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Munoz-Bertomeu, Jesus UNSPECIFIED UNSPECIFIED UNSPECIFIED Ros, Roc UNSPECIFIED orcid.org/0000-0003-4254-8368 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-239320
DOI:	10.1111/tpj.13452
Journal or Publication Title:	Plant J.
Volume:	89
Number:	6
Page Range:	S. 1146 - 1159
Date:	2017
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-313X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS-THALIANA; CARBOHYDRATE-METABOLISM; ENVELOPE MEMBRANE; PYRUVATE-KINASE; SEED; TRANSPORTERS; TISSUES; CARBON; GENE; IDENTIFICATION Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23932